Anti-EBNA2 [PE2]
Invented by Prof Martin Rowe from University Of Birmingham
Invented at University Of Birmingham
- Datasheet
- References (24)
- Inventor Info
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| Catalogue Number | 151472 |
| Applications | IHC IF IP WB ChIP-seq |
| Antigen/Gene or Protein Targets | Epstein Barr Virus Encoded Nuclear Antigen 2 (EBNA2) |
| Reactivity | Human and Virus |
| Relevance |
Monoclonal antibody directed at large T antigen of SV40, valuable for investigation into other SV40 tumour antigens and polyomaviruses. Background and Research Application The Epstein-Barr Virus (EBV), also called Human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. EBNA2 is one of the few genes of Epstein-Barr virus which are necessary for immortalization of human primary B lymphocytes. EBV is associated with several B-cell lymphomas and epithelial nasopharyngeal carcinoma. The EBNA2 protein acts as a transcriptional activator of several viral and cellular genes. There are two alternative forms of EBNA2: EBNA2A and EBNA2B. Anti-EBNA2 helped determine the subcellular localisation of the EBNA2 protein to the nuclei of transfected cells. This antibody recognises a shared epitope between the EBNA 2A and 2B proteins. EBNA2 expression in nasopharyngeal carcinoma biopsies was studied more extensively with anti-EBNA2. |
| Host | Mouse |
| Immunogen | Recombinant fusion protein against EBNA2The immunogen was a beta-gal fusion protein from plasmid pKH-2A10 that contains aa 341-480 of B95.8 type EBNA2A (Fig 4 in Young et al 1989) |
| Immunogen UniProt ID | P12978 |
| Positive Control | Immunohistochemistry (Acetone fixed): EBV positive Raji cell Line. Western blotting: B95-8 cell |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 52 |
| Myeloma Used | P3X63Ag8.653 |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-EBNA2 bind to both EBNA 2A and EBNA 2B proteins in immunoblotting and immunoprecipitation. Concentration 1mg/ml as standard |
| Research Area | Immunology, Virology |
References: 24 entries
Brocard et al. 2018. Nucleic Acids Res. 46(7):3707-3725. PMID: 29385536.
Wille et al. 2017. J Virol. 91(5):. PMID: 28003489.
David et al. 2017. Haematologica. :. PMID: 28232371.
WB
c-Myc dysregulation is a co-transforming event for nuclear factor-κB activated B cells.
Europe PMC ID: 28232371
Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency.
Europe PMC ID: 28003489
Wille et al. 2016. J Virol. :. PMID: 28003489.
WB
Wang et al. 2015. J Virol. :. PMID: 26719268.
WB ChIP-seq
Epstein-Barr Virus Nuclear Antigen 3 (EBNA3) Proteins Regulate EBNA2 Binding to Distinct RBPJ Genomic Sites.
Europe PMC ID: 26719268
Pujals et al. 2015. Autophagy. :. PMID: 26565591.
IHC WB
Constitutive autophagy contributes to resistance to TP53-mediated apoptosis in Epstein-Barr virus-positive latency III B-cell lymphoproliferations.
Europe PMC ID: 26565591
Gibson et al. 2011. Am J Surg Pathol. 35(6):807-15. PMID: 21552113.
EBV-positive extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue in the posttransplant setting: a distinct type of posttransplant lymphoproliferative disorder?
Europe PMC ID: 21552113
Boshoff et al. 1998. Blood. 91(5):1671-9. PMID: 9473233.
Establishing a KSHV+ cell line (BCP-1) from peripheral blood and characterizing its growth in Nod/SCID mice.
Europe PMC ID: 9473233
Brink et al. 1997. J Clin Pathol. 50(11):911-8. PMID: 9462239.
IHC WB
Presence of Epstein-Barr virus latency type III at the single cell level in post-transplantation lymphoproliferative disorders and AIDS related lymphomas.
Europe PMC ID: 9462239
Niedobitek et al. 1995. Blood. 86(2):659-65. PMID: 7605996.
IHC
Heterogeneous expression of Epstein-Barr virus latent proteins in endemic Burkitt's lymphoma.
Europe PMC ID: 7605996
Epstein-Barr virus (EBV)-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man.
Europe PMC ID: 1845872
Rowe et al. 1991. J Exp Med. 173(1):147-58. PMID: 1845872.
IF
Young et al. 1989. N Engl J Med. 321(16):1080-5. PMID: 2552313.
Expression of Epstein-Barr virus transformation-associated genes in tissues of patients with EBV lymphoproliferative disease.
Europe PMC ID: 2552313
Young et al. 1988. J Gen Virol. 69 ( Pt 5):1051-65. PMID: 2836550.
Epstein-Barr virus gene expression in nasopharyngeal carcinoma.
Europe PMC ID: 2836550
Add a reference
References: 24 entries
Brocard et al. 2018. Nucleic Acids Res. 46(7):3707-3725. PMID: 29385536.
Wille et al. 2017. J Virol. 91(5):. PMID: 28003489.
David et al. 2017. Haematologica. :. PMID: 28232371.
WB
c-Myc dysregulation is a co-transforming event for nuclear factor-κB activated B cells.
Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency.
Wille et al. 2016. J Virol. :. PMID: 28003489.
WB
Wang et al. 2015. J Virol. :. PMID: 26719268.
WB ChIP-seq
Epstein-Barr Virus Nuclear Antigen 3 (EBNA3) Proteins Regulate EBNA2 Binding to Distinct RBPJ Genomic Sites.
Pujals et al. 2015. Autophagy. :. PMID: 26565591.
IHC WB
Constitutive autophagy contributes to resistance to TP53-mediated apoptosis in Epstein-Barr virus-positive latency III B-cell lymphoproliferations.
Gibson et al. 2011. Am J Surg Pathol. 35(6):807-15. PMID: 21552113.
EBV-positive extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue in the posttransplant setting: a distinct type of posttransplant lymphoproliferative disorder?
Boshoff et al. 1998. Blood. 91(5):1671-9. PMID: 9473233.
Establishing a KSHV+ cell line (BCP-1) from peripheral blood and characterizing its growth in Nod/SCID mice.
Brink et al. 1997. J Clin Pathol. 50(11):911-8. PMID: 9462239.
IHC WB
Presence of Epstein-Barr virus latency type III at the single cell level in post-transplantation lymphoproliferative disorders and AIDS related lymphomas.
Niedobitek et al. 1995. Blood. 86(2):659-65. PMID: 7605996.
IHC
Heterogeneous expression of Epstein-Barr virus latent proteins in endemic Burkitt's lymphoma.
Epstein-Barr virus (EBV)-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man.
Rowe et al. 1991. J Exp Med. 173(1):147-58. PMID: 1845872.
IF
Young et al. 1989. N Engl J Med. 321(16):1080-5. PMID: 2552313.
Expression of Epstein-Barr virus transformation-associated genes in tissues of patients with EBV lymphoproliferative disease.
Young et al. 1988. J Gen Virol. 69 ( Pt 5):1051-65. PMID: 2836550.
Epstein-Barr virus gene expression in nasopharyngeal carcinoma.
Add a reference
