Anti-CD79a [HM47]
Invented by Dr Jacqueline Cordell from University of Oxford
Invented at University of Oxford
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151468 |
| Applications | IHC IP WB |
| Antigen/Gene or Protein Targets | CD79a |
| Synonyms | B-cell antigen receptor complex-associated protein alpha chain; Ig-alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein |
| Reactivity | Bovine, Chicken, Horse, Human, Guinea Pig, Mouse, Opossum, Pig, Primate, Rat and Rabbit |
| Relevance | The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections. |
| Host | Mouse |
| Immunogen | Synthetic peptide of 14 amino acids representing amino acids 202-216 of the human mb-1 cDNA sequence |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well. |
| Research Area | Adhesion, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology |
References: 8 entries
Lankester et al. 1994. J Immunol. 152(5):2157-62. PMID: 8133032.
Evidence for a direct physical interaction of membrane IgM, IgD, and IgG with the B29 gene product.
Europe PMC ID: 8133032
Brouns et al. 1993. Eur J Immunol. 23(5):1088-97. PMID: 8477803.
The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction.
Europe PMC ID: 8477803
Mason et al. 1991. J Immunol. 147(11):2474-82. PMID: 1747162.
The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
Europe PMC ID: 1747162
van Noesel et al. 1991. J Immunol. 146(11):3881-8. PMID: 2033258.
The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene.
Europe PMC ID: 2033258
Add a reference
References: 8 entries
Lankester et al. 1994. J Immunol. 152(5):2157-62. PMID: 8133032.
Evidence for a direct physical interaction of membrane IgM, IgD, and IgG with the B29 gene product.
Brouns et al. 1993. Eur J Immunol. 23(5):1088-97. PMID: 8477803.
The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction.
Mason et al. 1991. J Immunol. 147(11):2474-82. PMID: 1747162.
The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
van Noesel et al. 1991. J Immunol. 146(11):3881-8. PMID: 2033258.
The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene.
Add a reference
