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Anti-Melphalan-modified DNA [Amp4/42]


Catalogue Number 151465
Applications ELISA IF
Antigen/Gene or Protein Targets Melphalan
Reactivity Human and Mouse
Relevance Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali. The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and Amp4/42 appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
Host Rat
Subclass IgG2b
Positive Control DNA/ melphalan alkylated DNA
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions DMEM + 10%FCS + Glu + Pen/Strep
Research Area Cancer

References: 6 entries

Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15;283(2):127-34.PMID: 12581733

Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420.

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