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Anti-Melphalan-modified DNA [Amp4/42]

Invented by Dr Michael Tilby at University of Newcastle upon Tyne

Info

Catalogue Number 151465
Applications ELISA IF
Antigen/Gene or Protein Targets Melphalan
Reactivity Human and Mouse
Relevance Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali. The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and Amp4/42 appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
Host Rat
Subclass IgG2b
Positive Control DNA/ melphalan alkylated DNA
Myeloma Used Y3.AG.1.2.3
Recommended Growing Conditions DMEM + 10%FCS + Glu + Pen/Strep
Research Area Cancer

References: 10 entries

Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15;283(2):127-34.PMID: 12581733

Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494.

Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response.

Europe PMC ID: 12130494

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Europe PMC ID: 11170510

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

A monofunctional derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.

Europe PMC ID: 9778312

Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420.

A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy.

Europe PMC ID: 7634420


Add a reference

References: 10 entries

Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15;283(2):127-34.PMID: 12581733

Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494.

Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response.

Europe PMC ID: 12130494

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Europe PMC ID: 11170510

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

A monofunctional derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.

Europe PMC ID: 9778312

Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420.

A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy.

Europe PMC ID: 7634420


Add a reference

References: 10 entries

Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15;283(2):127-34.PMID: 12581733

Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494.

Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.

A monofunctional derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.

Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420.

A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy.


Add a reference


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