#151465

Anti-Melphalan-modified DNA [Amp4/42]

Cat. #151465

Anti-Melphalan-modified DNA [Amp4/42]

Cat. #: 151465

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Melphalan

Class: Monoclonal

Application: ELISA ; IF

Reactivity: Human ; Mouse

Host: Rat

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Michael Tilby

Institute: The Institute of Cancer Research; Newcastle University

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Melphalan-modified DNA [Amp4/42]
  • Clone: Amp4/42
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Rat
  • Application: ELISA ; IF
  • Description: Antibody Amp4/42 recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali
  • Isotype: IgG2b
  • Myeloma used: Y3.AG.1.2.3
  • Recommended controls: DNA/ melphalan alkylated DNA

Target Details

  • Target: Melphalan
  • Tissue cell line specificity: DNA/ melphalan alkylated DNA
  • Target background: The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and Amp4/42 appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.

Applications

  • Application: ELISA ; IF

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

Related Tools

  • Related tools: Anti-Melphalan-modified DNA [MP5/73]

References

  • Frank AJ and Tilby MJ. Exp Cell Res. 2003 Feb 15
  • 283(2):127-34.PMID: 12581733
  • Buschfort-Papewalis et al. 2002. Blood. 100(3):845-53. PMID: 12130494.
  • Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response.
  • McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.
  • McCartney et al. 2001. Chem Res Toxicol. 14(1):71-81. PMID: 11170510.
  • Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.
  • Tilby et al. 1998. Chem Res Toxicol. 11(10):1162-8. PMID: 9778312.
  • A monoFn derivative of melphalan: preparation, DNA alkylation products, and determination of the specificity of monoclonal antibodies that recognize melphalan-DNA adducts.
  • Tilby et al. 1995. Carcinogenesis. 16(8):1895-901. PMID: 7634420.
  • A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy.