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Bmx Cre ERT2 Mouse


Catalogue Number 151454
Antigen/Gene or Protein Targets BMX, CreERT2
Disease Keywords Cancer; angiogenesis;
Relevance The estragen receptor (ERT2) under the Bone marrow x (Bmx) promoter (Bmx-Cre-ERT2) mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in arterial endothelial cells. The Bmx-Cre-ERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.

Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP flanked genes in endothelial cells.

Non-induced Bmx-Cre-ERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Bmx-Cre-ERT2 mice demonstrate high penetrance in endothelial cells (95%+), significantly higher than existing endothelial Cre models currently available.
Production Details A Bmx-Cre-ERT2 transgene vector, containing a genomic VECad promoter fragment fused to a Cre-ERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Bmx-Cre-ERT2 transgene.
Conditional Yes
Conditional Description Conditional Cre-ERT2 expression under Bmx promoter enabling tissue-specific recombinase in arterial endothelial cells; inducible Cre activity by treatment with hormone (tamoxifen) enabling inducible translocation of Cre-ERT2 to nucleus.
Mouse Genetic Background/Cross History C57BL/6J (n=5)
Zygosity Heterozygous
Research Area Cardiovascular, Developmental Biology, Genetic Studies Tools, Immunology, Neurobiology

References: 4 entries

Murphy et al. 2014. Proc Natl Acad Sci U S A. 111(50):18007-12. PMID: 25468970.

Noels et al. 2014. Arterioscler Thromb Vasc Biol. 34(6):1209-20. PMID: 24723559.

Schober et al. 2014. Nat Med. 20(4):368-76. PMID: 24584117.

Ehling et al. 2013. Development. 140(14):3051-61. PMID: 23785053.

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Genotyping method PCR
Method protocol Primers: Sense: Bmx-s7 aaa tac ctt cag ttt tca tct Antisense: CRE-as1 ttg cga acc tca tca ctc gtt PCR programme: 94°C 2:00 min 94°C 0:30min 60°C 0:45min x35 cycles 72°C 0:45min 72C 5:00min Expected fragment size: 770bp
Additional notes This protocol was submitted by Susanne Adams from Ralf Adam's research group.