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Anti-Integrin aX [BU15]

Info

Catalogue Number 151435
Applications FACS IHC IF IP
Antigen/Gene or Protein Targets Integrin alpha X (CD11C)
Reactivity Dog, Human and Primate
Relevance Monoclonal antibody directed against integrin aX, with capability in diagnosing hairy cell leukaemia.

Background and Research Application
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.
Integrin alpha X is found on hairy cell leukaemias, malignant histiocytes, on tissue macrophages, monocytes, activated neutrophils, monocyte derivative dendritic cells and some T-cell clones. Abnormal expression of Integrin αX is characteristic of hairy cell leukaemia (HCL) and requires activation of proto-oncogenes Ras and JunD. Proteins and DNA elements that influence transcription of Integrin αX include Sp1 and Sp1-like factors, AP-1 family, C/EBP, Oct-2 and PU.1.
BU15 has utility for the diagnosis of hairy cell leukaemia.
Host Mouse
Immunogen Dendritic cells of synovial fluid
Immunogen UniProt ID P20702
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-Integrin aX BU15 reacts with canine, human, and non-human primate CD11c, (the 150 kDa integrin aX chain). This antibody has exhibited blocking activity, with crossblocking studies showing that the BU15 clone recognises a different epitope to the 3.9 clone.

Concentration
1mg/ml as standard
Research Area Adhesion, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References

There are 25 reference entries for this reagent.

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References: 25 entries

McMichael AJ et al. (Ed) 1997. Leucocyte Typing III. ISBN 13 9780192615527

Russo et al. 2019. J Neuroinflammation. 16(1):63. PMID: 30885223.

Wiedemann et al. 2019. Transl Oncol. 12(2):350-360. PMID: 30502589.

Langer et al. 2019. Elife. 8:. PMID: 30717826.

Gadalla et al. 2019. Front Oncol. 9:415. PMID: 31165047.

Maricic et al. 2018. J Immunol. 201(10):3017-3035. PMID: 30322964.

Stervbo et al. 2018. PLoS One. 13(11):e0206272. PMID: 30427865.

Myou et al. 2002. Immunology. 107(1):77-85. PMID: 12225365.

Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2.

Europe PMC ID: 12225365

Rezzonico et al. 2001. Blood. 97(10):2932-40. PMID: 11342414.

Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB.

Europe PMC ID: 11342414

Yoshino et al. 2000. Exp Anim. 49(2):97-110. PMID: 10889948.

Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.

Europe PMC ID: 10889948

Baker et al. 1998. Blood. 92(8):2830-43. PMID: 9763568.

Prolonged phenotypic, functional, and molecular change in group I Burkitt lymphoma cells on short-term exposure to CD40 ligand.

Europe PMC ID: 9763568

Van der Vieren et al. 1995. Immunity. 3(6):683-90. PMID: 8777714.

A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3.

Europe PMC ID: 8777714

Stewart et al. 1994. Methods Cell Biol. 41:39-60. PMID: 7861972.

Cell preparation for the identification of leukocytes.

Europe PMC ID: 7861972

Trowald-Wigh et al. 1993. Vet Immunol Immunopathol. 38(3-4):297-310. PMID: 8291207.

Canine neutrophil adhesion proteins and Fc-receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry.

Europe PMC ID: 8291207

Larson et al. 1990. Immunol Rev. 114:181-217. PMID: 2196220.

Structure and function of leukocyte integrins.

Europe PMC ID: 2196220

Hogg et al. 1986. Eur J Immunol. 16(3):240-8. PMID: 3456894.

The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.

Europe PMC ID: 3456894


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References: 25 entries

McMichael AJ et al. (Ed) 1997. Leucocyte Typing III. ISBN 13 9780192615527

Russo et al. 2019. J Neuroinflammation. 16(1):63. PMID: 30885223.

Wiedemann et al. 2019. Transl Oncol. 12(2):350-360. PMID: 30502589.

Langer et al. 2019. Elife. 8:. PMID: 30717826.

Gadalla et al. 2019. Front Oncol. 9:415. PMID: 31165047.

Maricic et al. 2018. J Immunol. 201(10):3017-3035. PMID: 30322964.

Stervbo et al. 2018. PLoS One. 13(11):e0206272. PMID: 30427865.

Myou et al. 2002. Immunology. 107(1):77-85. PMID: 12225365.

Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2.

Rezzonico et al. 2001. Blood. 97(10):2932-40. PMID: 11342414.

Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB.

Yoshino et al. 2000. Exp Anim. 49(2):97-110. PMID: 10889948.

Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.

Baker et al. 1998. Blood. 92(8):2830-43. PMID: 9763568.

Prolonged phenotypic, functional, and molecular change in group I Burkitt lymphoma cells on short-term exposure to CD40 ligand.

Van der Vieren et al. 1995. Immunity. 3(6):683-90. PMID: 8777714.

A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3.

Stewart et al. 1994. Methods Cell Biol. 41:39-60. PMID: 7861972.

Cell preparation for the identification of leukocytes.

Trowald-Wigh et al. 1993. Vet Immunol Immunopathol. 38(3-4):297-310. PMID: 8291207.

Canine neutrophil adhesion proteins and Fc-receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry.

Larson et al. 1990. Immunol Rev. 114:181-217. PMID: 2196220.

Structure and function of leukocyte integrins.

Hogg et al. 1986. Eur J Immunol. 16(3):240-8. PMID: 3456894.

The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.


Add a reference

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