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Anti-CD86 [BU63]

Info

Catalogue Number 151428
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets CD86
Synonyms B7.2
Reactivity Human
Relevance CD86 is a 70 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily of cell surface receptors. CD86 is expressed on resting monocytes and dendritic cells, activated T and Natural Killer cells, and is rapidly induced on the surface of activated B-cells, suggesting that it is a major CD28 ligand and may play an important role for the co-stimulation of T-cells in the primary immune response. CD86 is also expressed by malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Host Mouse
Immunogen ARH 77 (B-lymphoblastoid cell line)
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS
Strain Balb/c
Notes Recommended for the inhibition of the mixed lymphocyte reaction (MLR) and the binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD 86 (B7-2).
Western Blot 1:1000

Flow Cytometry 5 ug/ml

Immunohistochemistry 1:10-1:2000

Immunohistochemistry-Frozen 1:200

Immunoprecipitation 1:50
Research Area Cell Type or Organelle Marker, Immunology, Stem Cell Biology

References: 19 entries

Jeffery et al. 2015. PLoS One. 10(7):e0131539. PMID: 26134669.

FACS IF

Vitamin D Antagonises the Suppressive Effect of Inflammatory Cytokines on CTLA-4 Expression and Regulatory Function.

Europe PMC ID: 26134669

Means et al. 2007. J Virol. 81(12):6573-83. PMID: 17409151.

IP

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.

Europe PMC ID: 17409151

Giguère et al. 2004. J Virol. 78(12):6222-32. PMID: 15163715.

Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.

Europe PMC ID: 15163715

O'Sullivan et al. 2002. J Immunol. 168(11):5491-8. PMID: 12023343.

CD40 ligation conditions dendritic cell antigen-presenting function through sustained activation of NF-kappaB.

Europe PMC ID: 12023343

Latour et al. 2001. J Immunol. 167(5):2547-54. PMID: 11509594.

Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.

Europe PMC ID: 11509594

Mauri et al. 1995. J Immunol. 155(1):118-27. PMID: 7541409.

Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules.

Europe PMC ID: 7541409

Schlossman SF et al. 1995. Leucocyte Typing V Oxford University Press.

Caux et al. 1994. J Exp Med. 180(5):1841-7. PMID: 7525840.

B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells.

Europe PMC ID: 7525840

Engel et al. 1994. Blood. 84(5):1402-7. PMID: 7520767.

The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.

Europe PMC ID: 7520767

Nozawa et al. 1993. J Pathol. 169(3):309-15. PMID: 8492223.

A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.

Europe PMC ID: 8492223


Add a reference

References: 19 entries

Jeffery et al. 2015. PLoS One. 10(7):e0131539. PMID: 26134669.

FACS IF

Vitamin D Antagonises the Suppressive Effect of Inflammatory Cytokines on CTLA-4 Expression and Regulatory Function.

Europe PMC ID: 26134669

Means et al. 2007. J Virol. 81(12):6573-83. PMID: 17409151.

IP

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.

Europe PMC ID: 17409151

Giguère et al. 2004. J Virol. 78(12):6222-32. PMID: 15163715.

Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.

Europe PMC ID: 15163715

O'Sullivan et al. 2002. J Immunol. 168(11):5491-8. PMID: 12023343.

CD40 ligation conditions dendritic cell antigen-presenting function through sustained activation of NF-kappaB.

Europe PMC ID: 12023343

Latour et al. 2001. J Immunol. 167(5):2547-54. PMID: 11509594.

Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.

Europe PMC ID: 11509594

Mauri et al. 1995. J Immunol. 155(1):118-27. PMID: 7541409.

Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules.

Europe PMC ID: 7541409

Schlossman SF et al. 1995. Leucocyte Typing V Oxford University Press.

Caux et al. 1994. J Exp Med. 180(5):1841-7. PMID: 7525840.

B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells.

Europe PMC ID: 7525840

Engel et al. 1994. Blood. 84(5):1402-7. PMID: 7520767.

The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.

Europe PMC ID: 7520767

Nozawa et al. 1993. J Pathol. 169(3):309-15. PMID: 8492223.

A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.

Europe PMC ID: 8492223


Add a reference

References: 19 entries

Jeffery et al. 2015. PLoS One. 10(7):e0131539. PMID: 26134669.

FACS IF

Vitamin D Antagonises the Suppressive Effect of Inflammatory Cytokines on CTLA-4 Expression and Regulatory Function.

Means et al. 2007. J Virol. 81(12):6573-83. PMID: 17409151.

IP

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.

Giguère et al. 2004. J Virol. 78(12):6222-32. PMID: 15163715.

Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.

O'Sullivan et al. 2002. J Immunol. 168(11):5491-8. PMID: 12023343.

CD40 ligation conditions dendritic cell antigen-presenting function through sustained activation of NF-kappaB.

Latour et al. 2001. J Immunol. 167(5):2547-54. PMID: 11509594.

Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.

Mauri et al. 1995. J Immunol. 155(1):118-27. PMID: 7541409.

Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules.

Schlossman SF et al. 1995. Leucocyte Typing V Oxford University Press.

Caux et al. 1994. J Exp Med. 180(5):1841-7. PMID: 7525840.

B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells.

Engel et al. 1994. Blood. 84(5):1402-7. PMID: 7520767.

The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.

Nozawa et al. 1993. J Pathol. 169(3):309-15. PMID: 8492223.

A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.


Add a reference


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