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Anti-CD86 [BU63]

Info

Catalogue Number 151428
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets CD86
Synonyms B7.2
Reactivity Human
Relevance Monoclonal antibody directed against CD86 glycoprotein, without impacting on CTLA-4 activity. It aids the investigation of the adaptive immune response system.

Background and Research Application
CD86 is a 70 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily of cell surface receptors. CD86 was established during the 5th International Leukocyte Differentiation Antigen Workshop. CD86 is expressed on resting monocytes and dendritic cells, activated T and Natural Killer cells.
CD86 rapidly induced on the surface of activated B-cells and is the ligand for two different proteins on the T cell surface: CD28, for autoregulation and intercellular association, and CTLA-4, for attenuation of regulation and cellular disassociation. CD86 works in tandem with CD80 to prime T cells in the primary immune response. CD86 is also expressed by malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Anti-CD86 BU63, alongside another monoclonal antibody, helped define CD86 single-chain cell-surface protein expressed by blood monocytes and activated B cells. This antibody does not block CTLA-4 binding to B7-2/B70, but induces a conformational change in CD86 which increases the binding affinity of soluble CTLA-4.
Host Mouse
Immunogen ARH 77 (B-lymphoblastoid cell line)
Immunogen UniProt ID P42081
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-CD86 helped show that CD86 and B7-2B70 are the same molecule.

Recommended for the inhibition of the mixed lymphocyte reaction (MLR) and the binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD 86 (B7-2).
Western Blot 1:1000

Flow Cytometry 5 ug/ml

Immunohistochemistry 1:10-1:2000

Immunohistochemistry-Frozen 1:200

Immunoprecipitation 1:50

Concentration
1mg/ml as standard
Research Area Cell Type or Organelle Marker, Immunology, Stem Cell Biology

References

There are 20 reference entries for this reagent.

View All References

References: 20 entries

Santos et al. 2019. Immunology. 157(4):296-303. PMID: 31162836.

Jeffery et al. 2015. PLoS One. 10(7):e0131539. PMID: 26134669.

FACS IF

Vitamin D Antagonises the Suppressive Effect of Inflammatory Cytokines on CTLA-4 Expression and Regulatory Function.

Europe PMC ID: 26134669

Means et al. 2007. J Virol. 81(12):6573-83. PMID: 17409151.

IP

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.

Europe PMC ID: 17409151

Giguère et al. 2004. J Virol. 78(12):6222-32. PMID: 15163715.

Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.

Europe PMC ID: 15163715

O'Sullivan et al. 2002. J Immunol. 168(11):5491-8. PMID: 12023343.

CD40 ligation conditions dendritic cell antigen-presenting function through sustained activation of NF-kappaB.

Europe PMC ID: 12023343

Latour et al. 2001. J Immunol. 167(5):2547-54. PMID: 11509594.

Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.

Europe PMC ID: 11509594

Mauri et al. 1995. J Immunol. 155(1):118-27. PMID: 7541409.

Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules.

Europe PMC ID: 7541409

Schlossman SF et al. 1995. Leucocyte Typing V Oxford University Press.

Caux et al. 1994. J Exp Med. 180(5):1841-7. PMID: 7525840.

B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells.

Europe PMC ID: 7525840

Engel et al. 1994. Blood. 84(5):1402-7. PMID: 7520767.

The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.

Europe PMC ID: 7520767

Nozawa et al. 1993. J Pathol. 169(3):309-15. PMID: 8492223.

A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.

Europe PMC ID: 8492223


Add a reference

References: 20 entries

Santos et al. 2019. Immunology. 157(4):296-303. PMID: 31162836.

Jeffery et al. 2015. PLoS One. 10(7):e0131539. PMID: 26134669.

FACS IF

Vitamin D Antagonises the Suppressive Effect of Inflammatory Cytokines on CTLA-4 Expression and Regulatory Function.

Means et al. 2007. J Virol. 81(12):6573-83. PMID: 17409151.

IP

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.

Giguère et al. 2004. J Virol. 78(12):6222-32. PMID: 15163715.

Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle.

O'Sullivan et al. 2002. J Immunol. 168(11):5491-8. PMID: 12023343.

CD40 ligation conditions dendritic cell antigen-presenting function through sustained activation of NF-kappaB.

Latour et al. 2001. J Immunol. 167(5):2547-54. PMID: 11509594.

Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation.

Mauri et al. 1995. J Immunol. 155(1):118-27. PMID: 7541409.

Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules.

Schlossman SF et al. 1995. Leucocyte Typing V Oxford University Press.

Caux et al. 1994. J Exp Med. 180(5):1841-7. PMID: 7525840.

B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells.

Engel et al. 1994. Blood. 84(5):1402-7. PMID: 7520767.

The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.

Nozawa et al. 1993. J Pathol. 169(3):309-15. PMID: 8492223.

A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.


Add a reference

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