Anti-Spastin [Sp 6C6]
Invented by Giampietro Schiavo from University College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151424 |
| Applications | ELISA IF WB |
| Antigen/Gene or Protein Targets | Spastin |
| Reactivity | Human, Mouse and Rat |
| Relevance |
Monoclonal antibody for investigation into hereditary spastic paraplegias. Background and Research Application Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Spastin is involved in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutations within spastin cause the most common form of autosomal dominant spastic paraplegia 4. Mutant forms of spastin are generally found throughout the cytoplasm rather than within the nucleus. There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein, coded by the SPAST gene. |
| Host | Mouse |
| Immunogen | Recombinant Spastin |
| Immunogen UniProt ID | Q9UBP0 |
| Positive Control | HeLa cell or rat brain extract |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 52 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Suggested dilution for western blot: 1:500 Concentration 1mg/ml as standard |
| Research Area | Cardiovascular, Cell Cycle, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Metabolism, Neurobiology |
References: 2 entries
Salinas et al. 2005. J Neurochem. 95(5):1411-20. PMID: 16219033.
Human spastin has multiple microtubule-related functions.
Europe PMC ID: 16219033
Add a reference
References: 2 entries
Salinas et al. 2005. J Neurochem. 95(5):1411-20. PMID: 16219033.
Human spastin has multiple microtubule-related functions.
Add a reference
