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Anti-Ufd1 [5e2]


Catalogue Number 151414
Applications ELISA IF IP WB
Antigen/Gene or Protein Targets Ufd1
Reactivity Human, Rat, Mouse and Xenopus laevis
Relevance The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.
Host Mouse
Immunogen Full length mouse Ufd1 expressed in E. coli
Subclass IgG2a
Positive Control HeLa or HEK293 cell lysate.
Molecular Weight (kDa) 40
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 10% FCS.
Strain Balb/c
Notes Recommended Concentrations:

Western Blot: 0.5 ug/ml

Immunofluorescence: 5 ug/ml, (formaldehyde-fixed)
Research Area Cancer, Cell Cycle

References: 3 entries

Cao et al. 2003. Cell. 115(3):355-67. PMID: 14636562.

Hetzer et al. 2001. Nat Cell Biol. 3(12):1086-91. PMID: 11781570.

Meyer et al. 2000. EMBO J. 19(10):2181-92. PMID: 10811609.

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