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Anti-Spectrin [RG/26]


Catalogue Number 151403
Applications IHC IF IP WB
Antigen/Gene or Protein Targets Spectrin
Reactivity Human
Relevance Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.
Host Mouse
Immunogen Red cell membranes
Subclass IgG2a
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cardiovascular, Neurobiology, Protein Degradation

References: 2 entries

Wagner et al. 2014. Vox Sang. 106(1):45-54. PMID: 23909508.


Sachanonta et al. 2008. Asian Pac J Allergy Immunol. 26(1):37-45. PMID: 18595528.


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