Anti-HRX [HRX107]
Invented by Dr Jacqueline Cordell from University of Oxford
Invented at University of Oxford
- Datasheet
- References (4)
- Inventor Info
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![Adapted from Mueller et al. 2009. PLoS Biol. 7(11):e1000249. PMID: 19956800 Figure. Association of MLL fusion proteins with EAP in leukemia cells. (A) Coimmunoprecipitation (IP) of MLL-AF4 with endogenous ENL and CDK9 in patient-derived SEM cells. The MLL-AF4 fusion protein was precipitated by anti-MLL antibodies from extracts of the t(4;11) B-lymphocytic leukemia line SEM (upper panels). To control for precipitation with wild-type MLL, a B-ALL line of different etiology (REH) was included (lower panels). Precipitates were analyzed alongside a sample of input and probed for ENL and CDK9. Mock precipitations without antibody served as additional controls (contr). Note that MLL-AF4 corresponds in size to the N-terminal part of MLL that is produced after posttranslational cleavage of wild-type MLL. Therefore, MLL-AF4 and MLL-N comigrate as single band. In SEM as well as in REH, a longer splice variant of CDK9 (labeled by an asterisk [*]) also was prominently detectable.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/d5a84666-f6d2-470c-a734-cd0888981f2e.jpg)
![Adapted from Mueller et al. 2009. PLoS Biol. 7(11):e1000249. PMID: 19956800 Figure. Association of MLL fusion proteins with EAP in leukemia cells. (A) Coimmunoprecipitation (IP) of MLL-AF4 with endogenous ENL and CDK9 in patient-derived SEM cells. The MLL-AF4 fusion protein was precipitated by anti-MLL antibodies from extracts of the t(4;11) B-lymphocytic leukemia line SEM (upper panels). To control for precipitation with wild-type MLL, a B-ALL line of different etiology (REH) was included (lower panels). Precipitates were analyzed alongside a sample of input and probed for ENL and CDK9. Mock precipitations without antibody served as additional controls (contr). Note that MLL-AF4 corresponds in size to the N-terminal part of MLL that is produced after posttranslational cleavage of wild-type MLL. Therefore, MLL-AF4 and MLL-N comigrate as single band. In SEM as well as in REH, a longer splice variant of CDK9 (labeled by an asterisk [*]) also was prominently detectable.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/d5a84666-f6d2-470c-a734-cd0888981f2e.jpg)
![Image thumbnail for Anti-HRX [HRX107]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/d5a84666-f6d2-470c-a734-cd0888981f2e.jpg)
| Catalogue Number | 151384 |
| Applications | ChIP IHC IF WB |
| Antigen/Gene or Protein Targets | Histone-lysine N-methyltransferase (HRX, MLL, ALL1, Htrx) |
| Reactivity | Human |
| Relevance | The HRX gene (also called MLL, ALL-1 or Htrx) located to chromosome band 11q23 is associated with specific subsets of acute Lukaemia through translocations that that result in its fusion with a variety of heterologous partners |
| Host | Mouse |
| Immunogen | Synthetic peptides corresponding to 839-854 of the HRX protein |
| Subclass | IgM |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes | Ultroser G can be used at 1% if the cells are not growing well. |
| Research Area | Cancer, Epigenetics & Nuclear Signalling |
References: 4 entries
Mueller et al. 2009. PLoS Biol. 7(11):e1000249. PMID: 19956800.
Misguided transcriptional elongation causes mixed lineage leukemia.
Europe PMC ID: 19956800
Butler et al. 1997. Blood. 89(9):3361-70. PMID: 9129043.
The HRX proto-oncogene product is widely expressed in human tissues and localizes to nuclear structures.
Europe PMC ID: 9129043
Add a reference
References: 4 entries
Mueller et al. 2009. PLoS Biol. 7(11):e1000249. PMID: 19956800.
Misguided transcriptional elongation causes mixed lineage leukemia.
Butler et al. 1997. Blood. 89(9):3361-70. PMID: 9129043.
The HRX proto-oncogene product is widely expressed in human tissues and localizes to nuclear structures.
Add a reference
