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Anti-CD79a [JCB117]


Catalogue Number 151375
Applications IHC WB
Antigen/Gene or Protein Targets CD79a
Synonyms CD79a Molecule; Membrane-Bound Immunoglobulin-Associated Protein; Surface IgM-Associated Protein; MB-1 Membrane Glycoprotein; Ig-Alpha; IGA; CD79a Antigen; MB1
Reactivity Human
Relevance The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.
Host Mouse
Immunogen Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.

Western Blot (WB) 1:100-1:500

Immunohistochemistry (Paraffin)

(IHC (P)) 1:50-1:100

Flow Cytometry (FACS) 2 ug/test
Research Area Cancer, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References: 9 entries

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Delli et al. 2016. Ann Rheum Dis. :. PMID: 26757748.

Higashi et al. 2015. Leuk Lymphoma. :1-6. PMID: 25860238.

Radaev et al. 2010. Structure. 18(8):934-43. PMID: 20696394.

Boudova et al. 2006. J Cutan Pathol. 33(8):584-9. PMID: 16919035.

Boudova et al. 2005. Am J Dermatopathol. 27(5):375-86. PMID: 16148405.

Pilozzi et al. 1998. J Pathol. 186(2):140-3. PMID: 9924428.

Mason et al. 1995. Blood. 86(4):1453-9. PMID: 7632952.

Korkolopoulou et al. 1994. Histopathology. 24(6):511-5. PMID: 7520411.

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