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|Applications||FACS IHC IP WB|
|Antigen/Gene or Protein Targets||CD79a (mb1)|
|Synonyms||CD79a Molecule; Membrane-Bound Immunoglobulin-Associated Protein; Surface IgM-Associated Protein; MB-1 Membrane Glycoprotein; Ig-Alpha; IGA; CD79a Antigen; MB1|
|Reactivity||Bovine, Chicken, Horse, Human, Guinea Pig, Mouse, Opossum, Pig, Primate, Rat and Rabbit|
|Relevance||The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.|
|Immunogen||Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence.|
|Recommended Growing Conditions||RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT|
|Notes||Ultroser G can be used at 1% if the cells are not growing well.|
|Research Area||Cancer, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology|
References: 5 entries
Mason et al. 1995. Blood. 86(4):1453-9. PMID: 7632952.
Jones et al. 1993. J Immunol. 150(12):5429-35. PMID: 8515069.
Mason et al. 1992. Eur J Immunol. 22(10):2753-6. PMID: 1396979.
Mason et al. 1991. J Immunol. 147(11):2474-82. PMID: 1747162.
van Noesel et al. 1991. J Immunol. 146(11):3881-8. PMID: 2033258.
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