Anti-CDw75 [ZB55]
Invented by Karen Pulford from University of Oxford
Invented at University of Oxford
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151373 |
| Applications | FACS IHC IF |
| Antigen/Gene or Protein Targets | CDw75 (CD75s) |
| Synonyms | ST6 Beta-Galactoside Alpha-2;6-Sialyltransferase; CMP-N-Acetylneuraminate-Beta-Galactosamide-Alpha-2;6-Sialyltransferase; B-Cell Antigen CD75, Alpha 2;6-ST; ST6GalI 3; SIAT1; CMP-N-Acetylneuraminate Beta-Galactosamide Alpha-2;6-Sialyltransferase; Sialyltransferase 1; ST6N |
| Reactivity | Human |
| Relevance | CDw75 is a human leukocyte cell-surface antigen expressed in mature and activated B cells but not in B cells at earlier stages of development or in plasma cells. ZB55 can be used for studying germinal centre-derived B cell lymphomas. |
| Host | Mouse |
| Immunogen | CD32 peptides - this is correct, please see Notes |
| Subclass | IgM |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes |
Ultroser G can be used at 1% if the cells are not growing well. The inventor has confirmed CD32 peptides were used as the immunogen although the hybridoma ultimately selected produces antibodies specific to CDw75. |
| Research Area | Cancer, Immunology |
References: 2 entries
Pulford K. et al. 1996. Immunocytochemical distribution of the CDw75 antigen. Tissue Antigens. Abstract. 48:371-85. DOI: 10.1111/j.1399-0039.1996.tb02657.x
Tedder TF. et al.1995. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 494. ISBN-13: 978-0198577980
Add a reference
References: 2 entries
Pulford K. et al. 1996. Immunocytochemical distribution of the CDw75 antigen. Tissue Antigens. Abstract. 48:371-85. DOI: 10.1111/j.1399-0039.1996.tb02657.x
Tedder TF. et al.1995. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 494. ISBN-13: 978-0198577980
Add a reference
