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Anti-CDw75 [ZB55]

Invented by Karen Pulford at University of Oxford

Info

Catalogue Number 151373
Applications FACS IHC IF
Antigen/Gene or Protein Targets CDw75 (CD75s)
Synonyms ST6 Beta-Galactoside Alpha-2;6-Sialyltransferase; CMP-N-Acetylneuraminate-Beta-Galactosamide-Alpha-2;6-Sialyltransferase; B-Cell Antigen CD75, Alpha 2;6-ST; ST6GalI 3; SIAT1; CMP-N-Acetylneuraminate Beta-Galactosamide Alpha-2;6-Sialyltransferase; Sialyltransferase 1; ST6N
Reactivity Human
Relevance CDw75 is a human leukocyte cell-surface antigen expressed in mature and activated B cells but not in B cells at earlier stages of development or in plasma cells. ZB55 can be used for studying germinal centre-derived B cell lymphomas.
Host Mouse
Immunogen CD32 peptides - this is correct, please see Notes
Subclass IgM
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.

The inventor has confirmed CD32 peptides were used as the immunogen although the hybridoma ultimately selected produces antibodies specific to CDw75.
Research Area Cancer, Immunology

References: 2 entries

Pulford K. et al. 1996. Immunocytochemical distribution of the CDw75 antigen. Tissue Antigens. Abstract. 48:371-85. DOI: 10.1111/j.1399-0039.1996.tb02657.x

Tedder TF. et al.1995. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 494. ISBN-13: 978-0198577980


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References: 2 entries

Pulford K. et al. 1996. Immunocytochemical distribution of the CDw75 antigen. Tissue Antigens. Abstract. 48:371-85. DOI: 10.1111/j.1399-0039.1996.tb02657.x

Tedder TF. et al.1995. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 494. ISBN-13: 978-0198577980


Add a reference

References: 2 entries

Pulford K. et al. 1996. Immunocytochemical distribution of the CDw75 antigen. Tissue Antigens. Abstract. 48:371-85. DOI: 10.1111/j.1399-0039.1996.tb02657.x

Tedder TF. et al.1995. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 494. ISBN-13: 978-0198577980


Add a reference


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