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Anti-HLADRCD74 [By2]


Catalogue Number 151372
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Human Leukocyte Antigen-DR locus (HLA-DR) associated invariant chain (CD74)
Synonyms CD74 Molecule; HLA-DR Antigens-Associated Invariant Chain; Gamma Chain Of Class II Antigens; Ia-Associated Invariant Chain; MHC HLA-DR Gamma Chain; DHLAG; P33; CD74 Antigen; Ia-GAMMA; HLADG
Reactivity Human
Relevance HLA-DR-associated invariant chain (CD74) plays a role in antigen presentation. In addition to its expression on antigen-presenting cells, CD74 is expressed by carcinomas of renal, lung, gastric, and thymic origin and by certain sarcomas. The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases. It has also been shown to have value as a gastric carcinoma marker.
Host Mouse
Immunogen B cell lymphoma cells
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 µg per 1 x 106 cells).
Research Area Immunology

References: 2 entries

Jones M et al. 1995. Workshop mAb B029 (By2) - a new B-cell marker for use in diagnostic pathology. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 710-11. ISBN-13: 978-0198577980

Mason D. et al (eds) CD Guide for CD74 (2002) Leucocyte Typing VII, Oxford University Press, Oxford and New York, p 825-6. ISBN-13: 978-0192632524

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