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Anti-CD68 [KP1]
RECENTLY UPDATED

Invented by Karen Pulford at University of Oxford

Info

Catalogue Number 151369
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets CD68
Synonyms CD68 Molecule; CD68 Antigen; Macrophage Antigen CD68; GP110; Scavenger Receptor Class D; Member; Scavenger Receptor Class D; SCARD1; LAMP4
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody which recognises most human macrophages, useful in studying macrophage disorders including both reactive and neoplastic states.

Background and Research Application
Anti-CD68 (KP1) monoclonal antibody recognises a fixation-resistant epitope in a wide variety of tissue macrophages (such as Kupffer cells germinal centre, splenic, and lamina propria macrophages), and in granulocyte precursors. Anti-CD68 (KP1) recognises most human macrophages in routinely processed tissue, where the target can be identified by immunocytochemical techniques.

CD68 is a 110-kD transmembrane glycoprotein which is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and favours binding to lectins through its heavily glycosylated extracellular domain. CD68 is also a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34.

CD68 is resistant to conventional fixatives such as formalin and B5, as such KP1 may be of practical importance for diagnostic pathologists. CD68 persists throughout cell maturation from the monocyte to the mature macrophage stage. The quantity of this antigen increases upon macrophages when cells are activated and mature indicated by a greater intensity of staining. KP1 is widely used to detect macrophages in paraffin embedded tissue sections.
Host Mouse
Immunogen Lysosomal contents of lung macrophages
Immunogen UniProt ID
RECENTLY UPDATED
P34810
Positive Control Human spleen tissue, Mouse brain tissue or human liver tissue (IHC); Human lung cancer (IF)
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM)
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody can be used to assess the number and distribution of tissue macrophages in pathological conditions (such as inflammation and neoplasia). It may also be of value in the diagnosis of malignant histiocytic tumours, helping distinguish between true histiocytic malignancies and high-grade neoplasms of lymphoid origin.
Anti-CD68 has broad reactivity with cells of the mononuclear phagocytic lineage. Interdigitating reticulum cells were unstained or showed limited cytoplasmic staining while Langerhans' cells and follicular dendritic reticulum cells were unreactive.

This antibody proves useful for studying disorders of the monocyte/macrophage system, including both reactive and neoplastic states.
Megakaryocytes and cells of erythroid lineage are not labelled by KPI in smears or sections of bone marrow. In the spleen KP1 antibody labelled macrophages in germinal centres and in red pulp. No staining of marginal zone macrophages. In the thymus KP1 stained both medullary and cortical macrophages. Langerhans' cells, interdigitating reticulum cells, and follicular dendritic cells were all unstained.

Ultroser G can be used at 1% if the cells are not growing well.

Concentration
1mg/ml as standard
Research Area Cell Type or Organelle Marker, Immunology, Stem Cell Biology

References

There are 37 reference entries for this reagent.

View All References

References: 37 entries
RECENTLY UPDATED

Fovet et al. 2019. EBioMedicine. 47:492-505. PMID: 31492559.

Malissen et al. 2019. Oncoimmunology. 8(12):e1665976. PMID: 31741766.

MacGregor et al. 2019. Oncoimmunology. 8(12):e1665460. PMID: 31741762.

Kothari et al. 2018. Brain Pathol. 28(6):806-821. PMID: 30062819.

Fodil et al. 2017. Nat Commun. 8(1):932. PMID: 29030607.

Wen et al. 2017. Cell Death Dis. 8(2):e2593. PMID: 28151470.

IHC

Adipocytes activate mitochondrial fatty acid oxidation and autophagy to promote tumor growth in colon cancer.

Europe PMC ID: 28151470

Hyafil et al. 2016. J Nucl Med. :. PMID: 27789718.

IHC

Imaging the Cytokine Receptor CXCR4 in Atherosclerotic Plaques with the Radiotracer 68Ga-Pentixafor for PET.

Europe PMC ID: 27789718

Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?

Europe PMC ID: 27404454

Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.

IHC

Sugimoto et al. 2016. J Clin Exp Hematop. 56(1):1-19. PMID: 27334853.

Karamese et al. 2016. Immunopharmacol Immunotoxicol. 38(3):228-37. PMID: 27144896.

IHC

Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study.

Europe PMC ID: 27144896

α4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.

Europe PMC ID: 27157989

Lakritz et al. 2016. Am J Pathol. :. PMID: 27157989.

IHC

Armstrong et al. 2016. Oncotarget. 7(7):7885-98. PMID: 26799286.

IHC

PTEN deficiency promotes macrophage infiltration and hypersensitivity of prostate cancer to IAP antagonist/radiation combination therapy.

Europe PMC ID: 26799286

Follicular Lymphoma: The Role of the Tumor Microenvironment in Prognosis.

Europe PMC ID: 27334853

Liu et al. 2015. Medicine (Baltimore). 94(39):e1631. PMID: 26426650.

Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.

Europe PMC ID: 26426650

Lakritz et al. 2015. Am J Pathol. :. PMID: 25956030.

IHC

Monocyte Traffic, Dorsal Root Ganglion Histopathology, and Loss of Intraepidermal Nerve Fiber Density in SIV Peripheral Neuropathy.

Europe PMC ID: 25956030

Zhou et al. 2013. Proc Natl Acad Sci U S A. 110(46):E4335-44. PMID: 24167262.

Fibrinogen-specific antibody induces abdominal aortic aneurysm in mice through complement lectin pathway activation.

Europe PMC ID: 24167262

Chamberlain et al. 2013. PLoS One. 8(8):e71631. PMID: 23936523.

Interleukin expression after injury and the effects of interleukin-1 receptor antagonist.

Europe PMC ID: 23936523

Kunisch et al. 2004. Ann Rheum Dis. 63(7):774-84. PMID: 15194571.

FACS

Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry.

Europe PMC ID: 15194571

Doussis et al. 1993. J Clin Pathol. 46(4):334-6. PMID: 7684403.

CD68 reactivity of non-macrophage derived tumours in cytological specimens.

Europe PMC ID: 7684403

Pulford et al. 1990. Int Immunol. 2(10):973-80. PMID: 2078523.

Distribution of the CD68 macrophage/myeloid associated antigen.

Europe PMC ID: 2078523

Micklem et al. 1989. Br J Haematol. 73(1):6-11. PMID: 2803980.

A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.

Europe PMC ID: 2803980

Pulford et al. 1989. J Clin Pathol. 42(4):414-21. PMID: 2654191.

KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.

Europe PMC ID: 2654191


Add a reference

References: 37 entries
RECENTLY UPDATED

Fovet et al. 2019. EBioMedicine. 47:492-505. PMID: 31492559.

Malissen et al. 2019. Oncoimmunology. 8(12):e1665976. PMID: 31741766.

MacGregor et al. 2019. Oncoimmunology. 8(12):e1665460. PMID: 31741762.

Kothari et al. 2018. Brain Pathol. 28(6):806-821. PMID: 30062819.

Fodil et al. 2017. Nat Commun. 8(1):932. PMID: 29030607.

Wen et al. 2017. Cell Death Dis. 8(2):e2593. PMID: 28151470.

IHC

Adipocytes activate mitochondrial fatty acid oxidation and autophagy to promote tumor growth in colon cancer.

Hyafil et al. 2016. J Nucl Med. :. PMID: 27789718.

IHC

Imaging the Cytokine Receptor CXCR4 in Atherosclerotic Plaques with the Radiotracer 68Ga-Pentixafor for PET.

Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?

Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.

IHC

Sugimoto et al. 2016. J Clin Exp Hematop. 56(1):1-19. PMID: 27334853.

Karamese et al. 2016. Immunopharmacol Immunotoxicol. 38(3):228-37. PMID: 27144896.

IHC

Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study.

α4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.

Lakritz et al. 2016. Am J Pathol. :. PMID: 27157989.

IHC

Armstrong et al. 2016. Oncotarget. 7(7):7885-98. PMID: 26799286.

IHC

PTEN deficiency promotes macrophage infiltration and hypersensitivity of prostate cancer to IAP antagonist/radiation combination therapy.

Follicular Lymphoma: The Role of the Tumor Microenvironment in Prognosis.

Liu et al. 2015. Medicine (Baltimore). 94(39):e1631. PMID: 26426650.

Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.

Lakritz et al. 2015. Am J Pathol. :. PMID: 25956030.

IHC

Monocyte Traffic, Dorsal Root Ganglion Histopathology, and Loss of Intraepidermal Nerve Fiber Density in SIV Peripheral Neuropathy.

Zhou et al. 2013. Proc Natl Acad Sci U S A. 110(46):E4335-44. PMID: 24167262.

Fibrinogen-specific antibody induces abdominal aortic aneurysm in mice through complement lectin pathway activation.

Chamberlain et al. 2013. PLoS One. 8(8):e71631. PMID: 23936523.

Interleukin expression after injury and the effects of interleukin-1 receptor antagonist.

Kunisch et al. 2004. Ann Rheum Dis. 63(7):774-84. PMID: 15194571.

FACS

Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry.

Doussis et al. 1993. J Clin Pathol. 46(4):334-6. PMID: 7684403.

CD68 reactivity of non-macrophage derived tumours in cytological specimens.

Pulford et al. 1990. Int Immunol. 2(10):973-80. PMID: 2078523.

Distribution of the CD68 macrophage/myeloid associated antigen.

Micklem et al. 1989. Br J Haematol. 73(1):6-11. PMID: 2803980.

A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.

Pulford et al. 1989. J Clin Pathol. 42(4):414-21. PMID: 2654191.

KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.


Add a reference