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Anti-CEACAM5&6 [By114]


Catalogue Number 151368
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Carcinoembryonic antigen-related cell adhesion molecules 5 & 6 (CEACAM5, CD66e; CEACAM6, CD66c)CD66 C and E subtypes (NCA)
Synonyms Carcinoembryonic Antigen Related Cell Adhesion Molecule 5 & 6; Meconium Antigen 100; CEA; Carcinoembryonic Antigen; CD66e & c
Reactivity Human
Relevance CEACAM is a member of the carcinoembryonic antigen family (a diagnostic marker for many cancers). Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. This antibody is useful in differentiating normal cells from neoplastic cells of granulocyte origin. CD66CE is useful in differentiating normal cells from neoplastic cells of granulocyte origin. By114 may be used to study Paroxysmal Nocturnal Hemoglobinuria (PNH).
Host Mouse
Immunogen B cell Lymphoma cells
Subclass IgG1 kappa
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
CEACAM5/6 (By114) is recommended for detection of CEACAM5 and CEACAM6 of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Research Area Cancer, Cell Type or Organelle Marker

References: 2 entries

Tooze et al. 1995. Exp Hematol. 23(14):1484-91. PMID: 8542935.

Mayne et al. 1993. Br J Haematol. 83(1):30-8. PMID: 8435334.

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