Anti-CEACAM5&6 [By114]
Invented by Karen Pulford from University of Oxford
Invented at University of Oxford
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151368 |
| Applications | FACS IHC IF IP WB |
| Antigen/Gene or Protein Targets | Carcinoembryonic antigen-related cell adhesion molecules 5 & 6 (CEACAM5, CD66e; CEACAM6, CD66c)CD66 C and E subtypes (NCA) |
| Synonyms | Carcinoembryonic Antigen Related Cell Adhesion Molecule 5 & 6; Meconium Antigen 100; CEA; Carcinoembryonic Antigen; CD66e & c |
| Reactivity | Human |
| Relevance | CEACAM is a member of the carcinoembryonic antigen family (a diagnostic marker for many cancers). Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. This antibody is useful in differentiating normal cells from neoplastic cells of granulocyte origin. CD66CE is useful in differentiating normal cells from neoplastic cells of granulocyte origin. By114 may be used to study Paroxysmal Nocturnal Hemoglobinuria (PNH). |
| Host | Mouse |
| Immunogen | B cell Lymphoma cells |
| Subclass | IgG1 kappa |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes |
Ultroser G can be used at 1% if the cells are not growing well. CEACAM5/6 (By114) is recommended for detection of CEACAM5 and CEACAM6 of human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500), immunohistochemistry (including paraffin embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). |
| Research Area | Cancer, Cell Type or Organelle Marker |
References: 4 entries
Tooze et al. 1995. Exp Hematol. 23(14):1484-91. PMID: 8542935.
The novel monoclonal antibody By114 helps detect the early emergence of a paroxysmal nocturnal hemoglobinuria clone in aplastic anemia.
Europe PMC ID: 8542935
Mayne et al. 1993. Br J Haematol. 83(1):30-8. PMID: 8435334.
Antibody By114 is selective for the 90 kD PI-linked component of the CD66 antigen: a new reagent for the study of paroxysmal nocturnal haemoglobinuria.
Europe PMC ID: 8435334
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References: 4 entries
Tooze et al. 1995. Exp Hematol. 23(14):1484-91. PMID: 8542935.
The novel monoclonal antibody By114 helps detect the early emergence of a paroxysmal nocturnal hemoglobinuria clone in aplastic anemia.
Mayne et al. 1993. Br J Haematol. 83(1):30-8. PMID: 8435334.
Antibody By114 is selective for the 90 kD PI-linked component of the CD66 antigen: a new reagent for the study of paroxysmal nocturnal haemoglobinuria.
Add a reference
