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Anti-CD31 (PECAM1) [JC70]
RECENTLY UPDATED

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151357
Applications FACS IHC IF WB
Antigen/Gene or Protein Targets PECAM1 (CD31)
Synonyms Platelet/Endothelial Cell Adhesion Molecule 1; CD31 Antigen; EndoCAM; PECAM-1; GPIIA; PECA1; CD31/EndoCAM; CD31
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody directed against endothelial cell marker (CD31, JC70), capable of evaluating tumour angiogenesis and angiosarcoma more effectively than anti-FVIII-Reg.

Background and Research Application
CD31/JC70, also known as platelet endothelium cell adhesion molecule (PECAM-1), is a 130kDa transmembrane glycoprotein found on cell surface of endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. PECAM1 is a member of the immunoglobulin (Ig) superfamily. It is also a major constituent of the endothelial cell intercellular junction and expressed in certain cancers. PECAM1 is involved in cell signalling and adhesion, trans-endothelial migration of leukocytes, angiogenesis, and integrin activation. PECAM1 is a specific marker of endothelial differentiation.
Anti-CD31 be used to identify the presence of endothelial cells in a histological tissue sample to determine the degree of angiogenesis (which in turn can determine tumour severity/growth).
Anti-CD31 stains a fixation resistant epitope on endothelial cells in both benign and malignant endothelial cells in angiosarcomas with more consistency than monoclonal or polyclonal antibodies to FV111-Rag . CD31 is a good marker of endothelial cells in paraffin sections.
Anti-CD31 was created to recognise membrane bound glycoprotein CD31/JC70.
Host Mouse
Immunogen Cell membrane preparation from the spleen of a patient with hairy cell leukaemia
Immunogen UniProt ID
RECENTLY UPDATED
P16284
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM)
Notes
RECENTLY UPDATED
Original Antibody Development
BALB/c mice were intraperitoneally immunised with 50ug of cell membrane preparation from the spleen of a patient with hairy cell leukaemia in physiological saline at 10-day intervals. Splenocytes were fused with P3/NS1/1-Ag4.myeloma cells.

Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-CD31 is useful in studying benign and malignant human vascular disorders in processed tissue samples. It reacts with endothelial cells in a wide range of tissues, with specific reactions to endothelium when staining fixed tissue. Anti-CD31 allows for analysis of cutaneous angiosarcomas. Anti-CD31 is more evenly distributed and reliable than commonly used marker factor VIII antibody, used as a vascular endothelial cell marker.

Ultroser G can be used at 1% if the cells are not growing well.
Immunocytochemistry (ICC) 1:10-1:100
Immunofluorescence (IF) 1:10-1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:100
Flow Cytometry (FACS) 1:2
Western Blot (WB) 1:1-1:100

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Stem Cell Biology

References

There are 17 reference entries for this reagent.

View All References

References: 17 entries
RECENTLY UPDATED

Muller WA, Kishimoto T, et al (eds) 1997. Leucocyte Typing VI, Garland Publishing Inc., New York and London, p 362-364. ISBN-13: 978-0815327455

Thomson et al. 2018. Methods Mol Biol. 1846:153-160. PMID: 30242758.

Ceasrine et al. 2018. Elife. 7:. PMID: 30303066.

Frye et al. 2018. Nat Commun. 9(1):1511. PMID: 29666442.

Fujita et al. 2017. PLoS One. 12(9):e0184534. PMID: 28886194.

Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?

Europe PMC ID: 27404454

Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.

Europe PMC ID: 25961170

Zhao et al. 2013. Cancer Res. 73(20):6149-63. PMID: 24097821.

Novel modeling of cancer cell signaling pathways enables systematic drug repositioning for distinct breast cancer metastases.

Europe PMC ID: 24097821

Ding et al. 2013. PLoS One. 8(5):e63628. PMID: 23675495.

HIF-1α transgenic bone marrow cells can promote tissue repair in cases of corticosteroid-induced osteonecrosis of the femoral head in rabbits.

Europe PMC ID: 23675495

Orecchia et al. 2011. PLoS One. 6(9):e24307. PMID: 21931678.

Sirtinol treatment reduces inflammation in human dermal microvascular endothelial cells.

Europe PMC ID: 21931678

Parums et al. 1990. J Clin Pathol. 43(9):752-7. PMID: 2212067.

JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.

Europe PMC ID: 2212067


Add a reference

References: 17 entries
RECENTLY UPDATED

Muller WA, Kishimoto T, et al (eds) 1997. Leucocyte Typing VI, Garland Publishing Inc., New York and London, p 362-364. ISBN-13: 978-0815327455

Thomson et al. 2018. Methods Mol Biol. 1846:153-160. PMID: 30242758.

Ceasrine et al. 2018. Elife. 7:. PMID: 30303066.

Frye et al. 2018. Nat Commun. 9(1):1511. PMID: 29666442.

Fujita et al. 2017. PLoS One. 12(9):e0184534. PMID: 28886194.

Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?

Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.

Zhao et al. 2013. Cancer Res. 73(20):6149-63. PMID: 24097821.

Novel modeling of cancer cell signaling pathways enables systematic drug repositioning for distinct breast cancer metastases.

Ding et al. 2013. PLoS One. 8(5):e63628. PMID: 23675495.

HIF-1α transgenic bone marrow cells can promote tissue repair in cases of corticosteroid-induced osteonecrosis of the femoral head in rabbits.

Orecchia et al. 2011. PLoS One. 6(9):e24307. PMID: 21931678.

Sirtinol treatment reduces inflammation in human dermal microvascular endothelial cells.

Parums et al. 1990. J Clin Pathol. 43(9):752-7. PMID: 2212067.

JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.


Add a reference