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Anti-LewisX [By87a]


Catalogue Number 151350
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Lewis X (CD15)
Synonyms Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; ELAM-1 Ligand Fucosyltransferase; FUC-TIV; FCT3A; ELFT; Stage-Specific Embryonic Antigen; Alpha (1,3) Fucosyltransferase; EC; Lewis X; SSEA-1; FUTIV; CD15; LeX
Reactivity Human
Relevance Lewis X (CD15) is a branched pentasaccharide found on neutrophils, eosinophils and monocytes. Lewis X is distributed abnormally in myeloid leukeamias. Lewis X is commonly used in the diagnosis of Hodgkin's disease. It can also be used for analysis of myeloid leukaemias and the study of myeloid differentiation.
Host Mouse
Immunogen B cell lymphoma cells
Subclass IgM
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cancer, Immunology

References: 1 entry

Dorfman et al. 1986. Am J Pathol. 123(3):508-19. PMID: 3717303.

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