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Anti-SIGLEC2 [4KB128]

Invented by Karen Pulford at University of Oxford

Info

Catalogue Number 151339
Applications FACS
Antigen/Gene or Protein Targets Sialic acid binding Ig-like lectin 2 (SIGLEC2, CD22, BL-CAM)
Reactivity Human
Relevance SIGLEC-2 is first expressed in the cytomplasm of pre- and pro- B cells. SIGLEC-2 is broadly expressed in normal and neoplastic B cells and absent from other leucocytes and tissues. SIGLEC-2 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. SIGLEC-2 is strongly expressed in hairy cell leukaemias.
Host Mouse
Immunogen B cell lymphoma cells
Subclass IgG1 kappa
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cancer, Immunology, Stem Cell Biology

References: 6 entries

Law et al. 1994. Immunol Today. 15(9):442-9. PMID: 7945784.

Regulation of lymphocyte activation by the cell-surface molecule CD22.

Europe PMC ID: 7945784

Mason et al. 1987. Blood. 69(3):836-40. PMID: 3101766.

Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells.

Europe PMC ID: 3101766

Campana et al. 1985. J Immunol. 134(3):1524-30. PMID: 3918103.

Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue.

Europe PMC ID: 3918103


Add a reference

References: 6 entries

Law et al. 1994. Immunol Today. 15(9):442-9. PMID: 7945784.

Regulation of lymphocyte activation by the cell-surface molecule CD22.

Europe PMC ID: 7945784

Mason et al. 1987. Blood. 69(3):836-40. PMID: 3101766.

Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells.

Europe PMC ID: 3101766

Campana et al. 1985. J Immunol. 134(3):1524-30. PMID: 3918103.

Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue.

Europe PMC ID: 3918103


Add a reference

References: 6 entries

Law et al. 1994. Immunol Today. 15(9):442-9. PMID: 7945784.

Regulation of lymphocyte activation by the cell-surface molecule CD22.

Mason et al. 1987. Blood. 69(3):836-40. PMID: 3101766.

Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells.

Campana et al. 1985. J Immunol. 134(3):1524-30. PMID: 3918103.

Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue.


Add a reference


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