Anti-Biotin [BK-1/39]
Invented by Dr Jacqueline Cordell from University of Oxford
Invented at University of Oxford
- Datasheet
- References (19)
- Inventor Info
Info
| Catalogue Number | 151338 |
| Applications | IHC |
| Antigen/Gene or Protein Targets | Biotin |
| Reactivity | Human |
| Relevance | Anti-Biotin antibodies can be used to detect both biotinylated proteins and nucleic acids. Biotin is a water-soluble vitamin, generally classified as a B-complex vitamin. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. |
| Host | Mouse |
| Immunogen | Biotin conjugated to keyhole limpet haemocyanin |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes | Ultroser G can be used at 1% if the cells are not growing well. |
| Research Area | Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Epitope Tags, Metabolism |
References: 19 entries
de Lima et al. 2012. Braz J Microbiol. 43(1):393-404. PMID: 24031845.
IHC
Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection.
Europe PMC ID: 24031845
Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.
IHC
Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.
Cribriform-morular variant of papillary thyroid carcinoma--cytological and immunocytochemical findings of 18 cases.
Europe PMC ID: 20091902
Kojc et al. 2005. Hum Pathol. 36(1):16-21. PMID: 15712177.
IHC
Expression of CD34, alpha-smooth muscle actin, and transforming growth factor beta1 in squamous intraepithelial lesions and squamous cell carcinoma of the larynx and hypopharynx.
Europe PMC ID: 15712177
Kato et al. 2004. J Pathol. 203(3):814-21. PMID: 15221941.
IHC
Possible linkage between specific histological structures and aberrant reactivation of the Wnt pathway in adamantinomatous craniopharyngioma.
Europe PMC ID: 15221941
Xu et al. 2003. J Pathol. 199(1):58-67. PMID: 12474227.
IHC
Cribriform-morular variant of papillary thyroid carcinoma: a pathological and molecular genetic study with evidence of frequent somatic mutations in exon 3 of the beta-catenin gene.
Europe PMC ID: 12474227
Lu et al. 2000. J Oral Pathol Med. 29(9):445-51. PMID: 11016687.
IHC
Immunohistochemical study of the distribution of endogenous biotin and biotin-binding enzymes in ductal structures of salivary gland tumours.
Europe PMC ID: 11016687
Nakatani et al. 1998. Am J Surg Pathol. 22(4):399-411. PMID: 9537466.
IHC
Pulmonary adenocarcinomas of the fetal lung type: a clinicopathologic study indicating differences in histology, epidemiology, and natural history of low-grade and high-grade forms.
Europe PMC ID: 9537466
Umemura et al. 1996. Pathol Int. 46(2):122-9. PMID: 10846559.
IHC
'Magentosis' in human lactating breast: a mode of cell death accumulating single-stranded DNA stretches or breaks.
Europe PMC ID: 10846559
Arnholdt et al. 1991. Virchows Arch B Cell Pathol Incl Mol Pathol. 61(2):75-80. PMID: 1683723.
IHC
Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta.
Europe PMC ID: 1683723
Add a reference
References: 19 entries
de Lima et al. 2012. Braz J Microbiol. 43(1):393-404. PMID: 24031845.
IHC
Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection.
Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.
IHC
Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.
Cribriform-morular variant of papillary thyroid carcinoma--cytological and immunocytochemical findings of 18 cases.
Kojc et al. 2005. Hum Pathol. 36(1):16-21. PMID: 15712177.
IHC
Expression of CD34, alpha-smooth muscle actin, and transforming growth factor beta1 in squamous intraepithelial lesions and squamous cell carcinoma of the larynx and hypopharynx.
Kato et al. 2004. J Pathol. 203(3):814-21. PMID: 15221941.
IHC
Possible linkage between specific histological structures and aberrant reactivation of the Wnt pathway in adamantinomatous craniopharyngioma.
Xu et al. 2003. J Pathol. 199(1):58-67. PMID: 12474227.
IHC
Cribriform-morular variant of papillary thyroid carcinoma: a pathological and molecular genetic study with evidence of frequent somatic mutations in exon 3 of the beta-catenin gene.
Lu et al. 2000. J Oral Pathol Med. 29(9):445-51. PMID: 11016687.
IHC
Immunohistochemical study of the distribution of endogenous biotin and biotin-binding enzymes in ductal structures of salivary gland tumours.
Nakatani et al. 1998. Am J Surg Pathol. 22(4):399-411. PMID: 9537466.
IHC
Pulmonary adenocarcinomas of the fetal lung type: a clinicopathologic study indicating differences in histology, epidemiology, and natural history of low-grade and high-grade forms.
Umemura et al. 1996. Pathol Int. 46(2):122-9. PMID: 10846559.
IHC
'Magentosis' in human lactating breast: a mode of cell death accumulating single-stranded DNA stretches or breaks.
Arnholdt et al. 1991. Virchows Arch B Cell Pathol Incl Mol Pathol. 61(2):75-80. PMID: 1683723.
IHC
Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta.
Add a reference
