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Anti-Biotin [BK-1/39]

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151338
Applications IHC
Antigen/Gene or Protein Targets Biotin
Reactivity Human
Relevance Anti-Biotin antibodies can be used to detect both biotinylated proteins and nucleic acids. Biotin is a water-soluble vitamin, generally classified as a B-complex vitamin. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Host Mouse
Immunogen Biotin conjugated to keyhole limpet haemocyanin
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Epitope Tags, Metabolism

References: 19 entries

de Lima et al. 2012. Braz J Microbiol. 43(1):393-404. PMID: 24031845.

IHC

Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection.

Europe PMC ID: 24031845

Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.

IHC

Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.

Cribriform-morular variant of papillary thyroid carcinoma--cytological and immunocytochemical findings of 18 cases.

Europe PMC ID: 20091902

Kojc et al. 2005. Hum Pathol. 36(1):16-21. PMID: 15712177.

IHC

Expression of CD34, alpha-smooth muscle actin, and transforming growth factor beta1 in squamous intraepithelial lesions and squamous cell carcinoma of the larynx and hypopharynx.

Europe PMC ID: 15712177

Kato et al. 2004. J Pathol. 203(3):814-21. PMID: 15221941.

IHC

Possible linkage between specific histological structures and aberrant reactivation of the Wnt pathway in adamantinomatous craniopharyngioma.

Europe PMC ID: 15221941

Xu et al. 2003. J Pathol. 199(1):58-67. PMID: 12474227.

IHC

Cribriform-morular variant of papillary thyroid carcinoma: a pathological and molecular genetic study with evidence of frequent somatic mutations in exon 3 of the beta-catenin gene.

Europe PMC ID: 12474227

Lu et al. 2000. J Oral Pathol Med. 29(9):445-51. PMID: 11016687.

IHC

Immunohistochemical study of the distribution of endogenous biotin and biotin-binding enzymes in ductal structures of salivary gland tumours.

Europe PMC ID: 11016687

Nakatani et al. 1998. Am J Surg Pathol. 22(4):399-411. PMID: 9537466.

IHC

Pulmonary adenocarcinomas of the fetal lung type: a clinicopathologic study indicating differences in histology, epidemiology, and natural history of low-grade and high-grade forms.

Europe PMC ID: 9537466

Umemura et al. 1996. Pathol Int. 46(2):122-9. PMID: 10846559.

IHC

'Magentosis' in human lactating breast: a mode of cell death accumulating single-stranded DNA stretches or breaks.

Europe PMC ID: 10846559

Arnholdt et al. 1991. Virchows Arch B Cell Pathol Incl Mol Pathol. 61(2):75-80. PMID: 1683723.

IHC

Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta.

Europe PMC ID: 1683723


Add a reference

References: 19 entries

de Lima et al. 2012. Braz J Microbiol. 43(1):393-404. PMID: 24031845.

IHC

Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection.

Europe PMC ID: 24031845

Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.

IHC

Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.

Cribriform-morular variant of papillary thyroid carcinoma--cytological and immunocytochemical findings of 18 cases.

Europe PMC ID: 20091902

Kojc et al. 2005. Hum Pathol. 36(1):16-21. PMID: 15712177.

IHC

Expression of CD34, alpha-smooth muscle actin, and transforming growth factor beta1 in squamous intraepithelial lesions and squamous cell carcinoma of the larynx and hypopharynx.

Europe PMC ID: 15712177

Kato et al. 2004. J Pathol. 203(3):814-21. PMID: 15221941.

IHC

Possible linkage between specific histological structures and aberrant reactivation of the Wnt pathway in adamantinomatous craniopharyngioma.

Europe PMC ID: 15221941

Xu et al. 2003. J Pathol. 199(1):58-67. PMID: 12474227.

IHC

Cribriform-morular variant of papillary thyroid carcinoma: a pathological and molecular genetic study with evidence of frequent somatic mutations in exon 3 of the beta-catenin gene.

Europe PMC ID: 12474227

Lu et al. 2000. J Oral Pathol Med. 29(9):445-51. PMID: 11016687.

IHC

Immunohistochemical study of the distribution of endogenous biotin and biotin-binding enzymes in ductal structures of salivary gland tumours.

Europe PMC ID: 11016687

Nakatani et al. 1998. Am J Surg Pathol. 22(4):399-411. PMID: 9537466.

IHC

Pulmonary adenocarcinomas of the fetal lung type: a clinicopathologic study indicating differences in histology, epidemiology, and natural history of low-grade and high-grade forms.

Europe PMC ID: 9537466

Umemura et al. 1996. Pathol Int. 46(2):122-9. PMID: 10846559.

IHC

'Magentosis' in human lactating breast: a mode of cell death accumulating single-stranded DNA stretches or breaks.

Europe PMC ID: 10846559

Arnholdt et al. 1991. Virchows Arch B Cell Pathol Incl Mol Pathol. 61(2):75-80. PMID: 1683723.

IHC

Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta.

Europe PMC ID: 1683723


Add a reference

References: 19 entries

de Lima et al. 2012. Braz J Microbiol. 43(1):393-404. PMID: 24031845.

IHC

Epstein-Barr virus-associated gastric carcinoma in Brazil: comparison between in situ hybridization and polymerase chain reaction detection.

Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.

IHC

Hirokawa et al. 2010. Diagn Cytopathol. 38(12):890-6. PMID: 20091902.

Cribriform-morular variant of papillary thyroid carcinoma--cytological and immunocytochemical findings of 18 cases.

Kojc et al. 2005. Hum Pathol. 36(1):16-21. PMID: 15712177.

IHC

Expression of CD34, alpha-smooth muscle actin, and transforming growth factor beta1 in squamous intraepithelial lesions and squamous cell carcinoma of the larynx and hypopharynx.

Kato et al. 2004. J Pathol. 203(3):814-21. PMID: 15221941.

IHC

Possible linkage between specific histological structures and aberrant reactivation of the Wnt pathway in adamantinomatous craniopharyngioma.

Xu et al. 2003. J Pathol. 199(1):58-67. PMID: 12474227.

IHC

Cribriform-morular variant of papillary thyroid carcinoma: a pathological and molecular genetic study with evidence of frequent somatic mutations in exon 3 of the beta-catenin gene.

Lu et al. 2000. J Oral Pathol Med. 29(9):445-51. PMID: 11016687.

IHC

Immunohistochemical study of the distribution of endogenous biotin and biotin-binding enzymes in ductal structures of salivary gland tumours.

Nakatani et al. 1998. Am J Surg Pathol. 22(4):399-411. PMID: 9537466.

IHC

Pulmonary adenocarcinomas of the fetal lung type: a clinicopathologic study indicating differences in histology, epidemiology, and natural history of low-grade and high-grade forms.

Umemura et al. 1996. Pathol Int. 46(2):122-9. PMID: 10846559.

IHC

'Magentosis' in human lactating breast: a mode of cell death accumulating single-stranded DNA stretches or breaks.

Arnholdt et al. 1991. Virchows Arch B Cell Pathol Incl Mol Pathol. 61(2):75-80. PMID: 1683723.

IHC

Receptor-mediated processing of epidermal growth factor in the trophoblast of the human placenta.


Add a reference


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