Anti-Alkaline phosphatase [AP-7/6/7]
Invented by Dr Jacqueline Cordell from University of Oxford
Invented at University of Oxford
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151336 |
| Applications | IHC IF |
| Antigen/Gene or Protein Targets | Alkaline phosphatase |
| Reactivity | Bovine |
| Relevance | Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. |
| Host | Mouse |
| Immunogen | Calf intestinal alkaline phosphatase |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes |
AP-7/6/7 is recommended for APAAP immuno-alkaline phosphatase technique. Ultroser G can be used at 1% if the cells are not growing well. |
| Research Area | Cancer, Cell Cycle, Cell Type or Organelle Marker |
References: 2 entries
Cordell et al. 1984. J Histochem Cytochem. 32(2):219-29. PMID: 6198355.
Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).
Europe PMC ID: 6198355
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References: 2 entries
Cordell et al. 1984. J Histochem Cytochem. 32(2):219-29. PMID: 6198355.
Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes).
Add a reference
