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|Antigen/Gene or Protein Targets||Alkaline phosphatase|
|Relevance||Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.|
|Immunogen||Calf intestinal alkaline phosphatase|
|Recommended Growing Conditions||RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT|
AP-7/6/7 is recommended for APAAP immuno-alkaline phosphatase technique.
Ultroser G can be used at 1% if the cells are not growing well.
|Research Area||Cancer, Cell Cycle, Cell Type or Organelle Marker|
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