Anti-Spastin [Sp 3G11/1]
Invented by Giampietro Schiavo from University College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (7)
- Inventor Info
Info
| Catalogue Number | 151320 |
| Applications | ChIP ELISA IHC IF WB |
| Antigen/Gene or Protein Targets | Spastin |
| Reactivity | Human, Mouse and Rat |
| Relevance | Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus. There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. |
| Host | Mouse |
| Immunogen | Recombinant human spastin |
| Positive Control | HeLa cell or rat brain extract |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 52 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | Suggested dilution for western blot: 1:500 |
| Research Area | Cardiovascular, Cell Cycle, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Metabolism, Neurobiology |
References: 7 entries
Daftary et al. 2011. Mol Endocrinol. 25(9):1539-49. PMID: 21757506.
A novel role for the AAA ATPase spastin as a HOXA10 transcriptional corepressor in Ishikawa endometrial cells.
Europe PMC ID: 21757506
Lacroix et al. 2010. J Cell Biol. 189(6):945-54. PMID: 20530212.
Lacroix et al. 2010. J Cell Biol. 189(6):945-54. PMID: 20530212.
WB
Tubulin polyglutamylation stimulates spastin-mediated microtubule severing.
Europe PMC ID: 20530212
Salinas et al. 2005. J Neurochem. 95(5):1411-20. PMID: 16219033.
Human spastin has multiple microtubule-related functions.
Europe PMC ID: 16219033
Add a reference
References: 7 entries
Daftary et al. 2011. Mol Endocrinol. 25(9):1539-49. PMID: 21757506.
A novel role for the AAA ATPase spastin as a HOXA10 transcriptional corepressor in Ishikawa endometrial cells.
Lacroix et al. 2010. J Cell Biol. 189(6):945-54. PMID: 20530212.
Lacroix et al. 2010. J Cell Biol. 189(6):945-54. PMID: 20530212.
WB
Tubulin polyglutamylation stimulates spastin-mediated microtubule severing.
Salinas et al. 2005. J Neurochem. 95(5):1411-20. PMID: 16219033.
Human spastin has multiple microtubule-related functions.
Add a reference
