- References (2)
- Inventor Info
|FACS IHC IF IP
|Antigen/Gene or Protein Targets
|Cell division cycle protein 20 (cdc20)
Fizzy protein, CDC20, CDC20A, p55CDC, P55CDC-LSB
Human Entrez Gene ID 991
Human SwissProt Q12834
Human Unigene 524947
Human Gene Symbol CDC20
Human Chromosome Location 1p34.2
|Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
|Urea-denatured His6 human Cdc20 recombinant protein
|Ramos or HeLa cells. Tonsil or gastric carcinoma.
|Molecular Weight (kDa)
|Recommended Growing Conditions
|DMEM + 5% FCS
Cellular Localization: Cytoplasmic
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
|Cell Cycle, Protein Degradation
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