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Anti-Cdc20 [AR12.4]


Catalogue Number 151310
Applications FACS IHC IF IP
Antigen/Gene or Protein Targets Cell division cycle protein 20 (cdc20)
Synonyms Fizzy protein, CDC20, CDC20A, p55CDC, P55CDC-LSB
Human Entrez Gene ID 991
Human SwissProt Q12834
Human Unigene 524947
Human Gene Symbol CDC20
Human Chromosome Location 1p34.2
Reactivity Human
Relevance Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Host Mouse
Immunogen Urea-denatured His6 human Cdc20 recombinant protein
Subclass IgG1 kappa
Positive Control Ramos or HeLa cells. Tonsil or gastric carcinoma.
Molecular Weight (kDa) 55kDa
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Cellular Localization: Cytoplasmic
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Research Area Cell Cycle, Protein Degradation

References: 1 entry

Sironi et al. 2001. EMBO J. 20(22):6371-82. PMID: 11707408.

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