Anti-Cdc20 [AR12.4]
Invented at European Institute of Oncology
- Datasheet
- References (2)
- Inventor Info
Info
![Image thumbnail for Anti-Cdc20 [AR12.4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/a53b98da-8a5e-46a0-8ebf-042bf9d47120.jpg)
| Catalogue Number | 151310 |
| Applications | FACS IHC IF IP |
| Antigen/Gene or Protein Targets | Cell division cycle protein 20 (cdc20) |
| Synonyms |
Fizzy protein, CDC20, CDC20A, p55CDC, P55CDC-LSB Human Entrez Gene ID 991 Human SwissProt Q12834 Human Unigene 524947 Human Gene Symbol CDC20 Human Chromosome Location 1p34.2 |
| Reactivity | Human |
| Relevance | Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. |
| Host | Mouse |
| Immunogen | Urea-denatured His6 human Cdc20 recombinant protein |
| Positive Control | Ramos or HeLa cells. Tonsil or gastric carcinoma. |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 55kDa |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Cellular Localization: Cytoplasmic Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) |
| Research Area | Cell Cycle, Protein Degradation |
References: 2 entries
Sironi et al. 2001. EMBO J. 20(22):6371-82. PMID: 11707408.
Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.
Europe PMC ID: 11707408
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References: 2 entries
Sironi et al. 2001. EMBO J. 20(22):6371-82. PMID: 11707408.
Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.
Add a reference
