- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||Murine Double Minute 2 protein (MDM2), Phosphorylated (Serine 186)|
Monoclonal antibody which targets the critical ser186 residue for MDM2 function.
Background and Research Application
MDM2 is a ubiquitin ligase whose principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This promotes progression through the cell cycle and cell survival. MDM2 has been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumour suppressor function.
MDM2 is over-expressed in many tumours, including soft tissue carcinomas and breast cancer. The phosphorylation of serines 166 and 186 is necessary for MDM2's translocation into the nucleus, thereby facilitating interaction with p53. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumour and normal tissues.
This antibody is directed against serine 186, which is crucial for MDM2 function.
|Immunogen||Peptide QRKRHKS(p)DSIS. S(p) represent a phosphorylated serine. This peptide represents the portion of MDM2 around Serine 186|
|Immunogen UniProt ID||Q00987|
|Positive Control||Breast carcinoma|
|Molecular Weight (kDa)||90|
|Recommended Growing Conditions||DMEM + 5% FCS|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
1mg/ml as standard
|Research Area||Cancer, Cell Cycle, Epigenetics & Nuclear Signalling|
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