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|Applications||FACS IHC IF IP WB|
|Antigen/Gene or Protein Targets||Cyclin A|
CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2
Human Entrez Gene ID: 890
Human SwissProt: P20248
Human Unigene: 58974
Human Gene Symbol: CCNA2
Human Chromosome Location: 4q27
|Reactivity||Bovine, Human, Mink and Mouse|
|Relevance||The anti Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase. Few other monoclonal antibodies other than E67.1 are able to immunoprecipitate active CDK/Cyclin A H1 kinase from such a wide range of species.|
|Immunogen||Full length bovine Cyclin A protein|
|Positive Control||K-562 and HeLa Cells|
|Molecular Weight (kDa)||54kDa|
|Recommended Growing Conditions||DMEM + 5% FCS|
Cellular Localization: Predominantly nuclear
Suggested dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml);
|Research Area||Cancer, Cell Cycle, Epigenetics & Nuclear Signalling|
References: 6 entries
Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116.
Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932.
Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612.
Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
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