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Anti-MDM2 [SMP14]

Invented by David Lane at Karolinska Institutet

Info

Catalogue Number 151295
Applications ELISA IHC IF IP WB
Antigen/Gene or Protein Targets Murine Double Minute 2 protein (MDM2)
Reactivity Human
Relevance MDM2 is over-expressed in many tumours. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein.
Host Mouse
Immunogen Synthetic peptide CSRPSTSSRRRAISE (residues 154-167 in human MDM2) cross-linked to KLH using glutaldehyde.
Subclass IgG2a
Positive Control Breast carcinoma
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Research Area Adhesion, Cancer, Cell Cycle, Epigenetics & Nuclear Signalling

References: 16 entries

Tao et al. 2013. Cell Res. 23(5):620-34. PMID: 23357851.

WB

Jiang et al. 2013. Nature. 493(7434):689-93. PMID: 23334421.

WB

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.

Europe PMC ID: 23357851

Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.

Europe PMC ID: 23334421

Das et al. 2007. Int J Surg Pathol. 15(4):335-45. PMID: 17913939.

p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters.

Europe PMC ID: 17913939

Hashemi et al. 2002. Cancer Lett. 180(2):211-21. PMID: 12175554.

IF

A melanoma-predisposing germline CDKN2A mutation with functional significance for both p16 and p14ARF.

Europe PMC ID: 12175554

Zhang et al. 2001. J Biol Chem. 276(32):29702-10. PMID: 11359766.

ELISA WB

Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.

Europe PMC ID: 11359766

Böttger et al. 1997. J Mol Biol. 269(5):744-56. PMID: 9223638.

ELISA

Molecular characterization of the hdm2-p53 interaction.

Europe PMC ID: 9223638

Dowell et al. 1996. Cytopathology. 7(5):340-51. PMID: 8911758.

Expression of p21waf1/Cip1, MDM2 and p53 in vivo: analysis of cytological preparations.

Europe PMC ID: 8911758

Picksley et al. 1994. Oncogene. 9(9):2523-9. PMID: 8058315.

Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides.

Europe PMC ID: 8058315


Add a reference

References: 16 entries

Tao et al. 2013. Cell Res. 23(5):620-34. PMID: 23357851.

WB

Jiang et al. 2013. Nature. 493(7434):689-93. PMID: 23334421.

WB

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.

Europe PMC ID: 23357851

Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.

Europe PMC ID: 23334421

Das et al. 2007. Int J Surg Pathol. 15(4):335-45. PMID: 17913939.

p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters.

Europe PMC ID: 17913939

Hashemi et al. 2002. Cancer Lett. 180(2):211-21. PMID: 12175554.

IF

A melanoma-predisposing germline CDKN2A mutation with functional significance for both p16 and p14ARF.

Europe PMC ID: 12175554

Zhang et al. 2001. J Biol Chem. 276(32):29702-10. PMID: 11359766.

ELISA WB

Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.

Europe PMC ID: 11359766

Böttger et al. 1997. J Mol Biol. 269(5):744-56. PMID: 9223638.

ELISA

Molecular characterization of the hdm2-p53 interaction.

Europe PMC ID: 9223638

Dowell et al. 1996. Cytopathology. 7(5):340-51. PMID: 8911758.

Expression of p21waf1/Cip1, MDM2 and p53 in vivo: analysis of cytological preparations.

Europe PMC ID: 8911758

Picksley et al. 1994. Oncogene. 9(9):2523-9. PMID: 8058315.

Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides.

Europe PMC ID: 8058315


Add a reference

References: 16 entries

Tao et al. 2013. Cell Res. 23(5):620-34. PMID: 23357851.

WB

Jiang et al. 2013. Nature. 493(7434):689-93. PMID: 23334421.

WB

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.

Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.

Das et al. 2007. Int J Surg Pathol. 15(4):335-45. PMID: 17913939.

p53 and mdm2 expression in colorectal carcinoma: a correlative analysis with clinical staging and histological parameters.

Hashemi et al. 2002. Cancer Lett. 180(2):211-21. PMID: 12175554.

IF

A melanoma-predisposing germline CDKN2A mutation with functional significance for both p16 and p14ARF.

Zhang et al. 2001. J Biol Chem. 276(32):29702-10. PMID: 11359766.

ELISA WB

Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.

Böttger et al. 1997. J Mol Biol. 269(5):744-56. PMID: 9223638.

ELISA

Molecular characterization of the hdm2-p53 interaction.

Dowell et al. 1996. Cytopathology. 7(5):340-51. PMID: 8911758.

Expression of p21waf1/Cip1, MDM2 and p53 in vivo: analysis of cytological preparations.

Picksley et al. 1994. Oncogene. 9(9):2523-9. PMID: 8058315.

Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides.


Add a reference


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