Anti-Rad51C [Rad51C 2H11/6]
Invented by Dr Stephen West from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 151286 |
| Applications | IHC IF IP WB |
| Antigen/Gene or Protein Targets | Rad51C |
| Reactivity | N/A |
| Relevance | RAD51C is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51C is also a component for the homologous recombination pathway. |
| Host | Mouse |
| Immunogen | Rad51C 2H11/6 was raised against His-tagged human Rad51C, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | Rad51C 2H11/6 detects as little as 10ng of purified protein and also detects endogenouse hamster Rad51C in whole V79 cell extracts by western blotting. |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 10 entries
Park et al. 2014. Oncogene. 33(40):4803-12. PMID: 24141787.
Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair.
Europe PMC ID: 24141787
French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
Europe PMC ID: 11912211
Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Europe PMC ID: 11751635
Masson et al. 2001. Proc Natl Acad Sci U S A. 98(15):8440-6. PMID: 11459987.
Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
Europe PMC ID: 11459987
Dosanjh et al. 1998. Nucleic Acids Res. 26(5):1179-84. PMID: 9469824.
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
Europe PMC ID: 9469824
Add a reference
References: 10 entries
Park et al. 2014. Oncogene. 33(40):4803-12. PMID: 24141787.
Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair.
French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability.
Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Masson et al. 2001. Proc Natl Acad Sci U S A. 98(15):8440-6. PMID: 11459987.
Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
Dosanjh et al. 1998. Nucleic Acids Res. 26(5):1179-84. PMID: 9469824.
Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.
Add a reference
