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|Applications||IHC IF IP WB|
|Antigen/Gene or Protein Targets||Rad51C|
|Relevance||RAD51C is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51C is also a component for the homologous recombination pathway.|
|Immunogen||Rad51C 2H11/6 was raised against His-tagged human Rad51C, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page|
|Recommended Growing Conditions||DMEM + 5% FCS|
|Notes||Rad51C 2H11/6 detects as little as 10ng of purified protein and also detects endogenouse hamster Rad51C in whole V79 cell extracts by western blotting.|
|Research Area||DNA Damage and Repair, Epigenetics & Nuclear Signalling|
References: 5 entries
Park et al. 2014. Oncogene. 33(40):4803-12. PMID: 24141787.
French et al. 2002. J Biol Chem. 277(22):19322-30. PMID: 11912211.
Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
Masson et al. 2001. Proc Natl Acad Sci U S A. 98(15):8440-6. PMID: 11459987.
Dosanjh et al. 1998. Nucleic Acids Res. 26(5):1179-84. PMID: 9469824.
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