Anti-Rad51D [Rad51D 5B3/6]
Invented by Dr Stephen West from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151269 |
| Applications | IP WB |
| Antigen/Gene or Protein Targets | Rad51D |
| Reactivity | Human |
| Relevance | RAD51D is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51D is also a component for the homologous recombination pathway. |
| Host | Mouse |
| Immunogen | His-tagged human Rad51D, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Detects as little as 10ng of purified protein. Dilutions Western Blot 1:1000 Immunocytochemistry/Immunofluorescence 1:100-1:500 |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 6 entries
Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Europe PMC ID: 11751635
Kawabata et al. 1999. Biochem Biophys Res Commun. 257(1):156-62. PMID: 10092526.
Multiple alternative transcripts of the human homologue of the mouse TRAD/R51H3/RAD51D gene, a member of the rec A/RAD51 gene family.
Europe PMC ID: 10092526
Pittman et al. 1998. Genomics. 49(1):103-11. PMID: 9570954.
Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene.
Europe PMC ID: 9570954
Add a reference
References: 6 entries
Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Kawabata et al. 1999. Biochem Biophys Res Commun. 257(1):156-62. PMID: 10092526.
Multiple alternative transcripts of the human homologue of the mouse TRAD/R51H3/RAD51D gene, a member of the rec A/RAD51 gene family.
Pittman et al. 1998. Genomics. 49(1):103-11. PMID: 9570954.
Identification, characterization, and genetic mapping of Rad51d, a new mouse and human RAD51/RecA-related gene.
Add a reference
