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Anti-Rad51D [Rad51D 5B3/6]

Invented by Dr Stephen West at Francis Crick Institute

Info

Catalogue Number 151269
Applications IP WB
Antigen/Gene or Protein Targets Rad51D
Reactivity Human
Relevance RAD51D is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51D is also a component for the homologous recombination pathway.
Host Mouse
Immunogen His-tagged human Rad51D, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Detects as little as 10ng of purified protein.
Dilutions Western Blot 1:1000
Immunocytochemistry/Immunofluorescence 1:100-1:500
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling

References: 3 entries

Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.

Kawabata et al. 1999. Biochem Biophys Res Commun. 257(1):156-62. PMID: 10092526.

Pittman et al. 1998. Genomics. 49(1):103-11. PMID: 9570954.


Add a reference

References: 3 entries

Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.

Kawabata et al. 1999. Biochem Biophys Res Commun. 257(1):156-62. PMID: 10092526.

Pittman et al. 1998. Genomics. 49(1):103-11. PMID: 9570954.


Add a reference

References: 3 entries

Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.

Kawabata et al. 1999. Biochem Biophys Res Commun. 257(1):156-62. PMID: 10092526.

Pittman et al. 1998. Genomics. 49(1):103-11. PMID: 9570954.


Add a reference


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