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Monoclonal Antibodies

Anti-Cul1 [AS97.1]

Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Adapted from Mao et al. 2011. J Biol Chem; 286(37):32355-65. PMID: 21778237. Figure C and D, Cul1 WT or a T47A point mutant fused to GST were expressed in HEK 293 cells. Their interactions with HA-Skp1 or FLAG-CAND1 were tested by co-precipitation.
Adapted from Ang et al. 2012. PLoS Biol. 10(3):e1001290. PMID: 22479149. Figure. Mediator interacts with SCF E3 ubiquitin ligases. Whole cell extracts of HeLa cells expressing myc-tagged hSkp1 (lanes 1 to 3) were incubated with anti-myc beads (lane 3). Whole cell extracts from untransfected HeLa cells (lanes 4 to 10) were incubated with an anti-hCul1 antibody (lanes 5 and 8) and with an anti-hMed6 antibody (lane 10). Antibodies were precipitated with Protein G-coupled beads. Inputs and precipitates were analyzed by Western blot with an anti-hMed6 antibody (lanes 1 to 5) and with an anti-hCul1 antibody (lanes 6 to 10).
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Catalogue Number 151253
Applications IP WB
Antigen/Gene or Protein Targets Cullin 1 (Cul1)
Reactivity Human and Mouse
Relevance Cul-1 is a subunit of the SCF (Skp1/Cul-1/F-box protein complex), an E3 ubiquitination ligase that is responsible for destruction of proteins during the S phase of the cell cycle.
Host Mouse
Immunogen Bacterially expressed full length recombinant human culllin 1. (Cul1)
Subclass IgG2a
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 10% FCS
Notes Recommended concentration: 2.5ug/ml
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle

References

There are 4 reference entries for this reagent.

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References: 4 entries

Ang et al. 2012. PLoS Biol. 10(3):e1001290. PMID: 22479149.

IP WB

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Mao et al. 2011. J Biol Chem. 286(37):32355-65. PMID: 21778237.

IP WB

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COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.

Europe PMC ID: 21778237

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Histone deacetylase inhibitor trichostatin A represses estrogen receptor alpha-dependent transcription and promotes proteasomal degradation of cyclin D1 in human breast carcinoma cell lines.

Europe PMC ID: 15585645

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References: 4 entries

Ang et al. 2012. PLoS Biol. 10(3):e1001290. PMID: 22479149.

IP WB

View  

Mao et al. 2011. J Biol Chem. 286(37):32355-65. PMID: 21778237.

IP WB

View  

COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.

View  

Histone deacetylase inhibitor trichostatin A represses estrogen receptor alpha-dependent transcription and promotes proteasomal degradation of cyclin D1 in human breast carcinoma cell lines.

View  

Add a reference

Inventor Information