Anti-CDKN2A/p14ARF [ARF 4C6/4]
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151246 |
| Applications | ELISA IHC IF IP WB |
| Antigen/Gene or Protein Targets | p14 ARF |
| Reactivity | Human |
| Relevance | p14ARF is associated with the regulation of p53 and MDM2 and is deleted or methylated in many human cancers. The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. |
| Host | Mouse |
| Immunogen | His-tagged p14ARF protein expressed in bacteria. |
| Positive Control | BT549 breast cancer cell line. |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 14 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling |
References: 8 entries
Ma et al. 2007. Mol Biol Cell. 18(7):2630-5. PMID: 17494866.
Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway.
Europe PMC ID: 17494866
p14ARF expression in invasive breast cancers and ductal carcinoma in situ--relationships to p53 and Hdm2.
Europe PMC ID: 15318938
Vestey et al. 2004. Breast Cancer Res. 6(5):R571-85. PMID: 15318938.
IHC
Clark et al. 2002. Oncogene. 21(29):4498-507. PMID: 12085228.
Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2.
Europe PMC ID: 12085228
Llanos et al. 2001. Nat Cell Biol. 3(5):445-52. PMID: 11331871.
IF
Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.
Europe PMC ID: 11331871
Add a reference
References: 8 entries
Ma et al. 2007. Mol Biol Cell. 18(7):2630-5. PMID: 17494866.
Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway.
p14ARF expression in invasive breast cancers and ductal carcinoma in situ--relationships to p53 and Hdm2.
Vestey et al. 2004. Breast Cancer Res. 6(5):R571-85. PMID: 15318938.
IHC
Clark et al. 2002. Oncogene. 21(29):4498-507. PMID: 12085228.
Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2.
Llanos et al. 2001. Nat Cell Biol. 3(5):445-52. PMID: 11331871.
IF
Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.
Add a reference
