Anti-PIP2 [PIP2 2C11]
Invented by Giampietro Schiavo from University College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151237 |
| Applications | ChIP ELISA IHC IF IP Fn DB |
| Antigen/Gene or Protein Targets | Phosphatidylinositol biphosphate (PIP2) |
| Reactivity | N/A |
| Relevance | PtdIns 4,5-P2 is a membrane phospholipid and an important component of signal transduction pathways. |
| Host | Mouse |
| Immunogen | Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2. |
| Positive Control | To test the Ab efficiency use a lipid blot assay as described in References above. |
| Subclass | IgM |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | It is recommended to use a lipid blot assay or nuclear staining to test this antibody. Cross reacts with PtdIns(4,5)P2, Ptd(4)P and Ptd(3,4,5)P3. |
| Research Area | Cell Signaling & Signal Transduction, Neurobiology |
References: 8 entries
Toska et al. 2014. Oncogene. 33(43):5100-8. PMID: 24166496.
Prohibitin is required for transcriptional repression by the WT1-BASP1 complex.
Europe PMC ID: 24166496
Ozkucur et al. 2011. BMC Cell Biol. 12:4. PMID: 21255452.
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
Europe PMC ID: 21255452
Osborne et al. 2001. J Cell Sci. 114(Pt 13):2501-11. PMID: 11559758.
Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing.
Europe PMC ID: 11559758
Thomas et al. 1999. Biochem Soc Trans. 27(4):648-52. PMID: 10917659.
Generation of phosphatidylinositol-specific antibodies and their characterization.
Europe PMC ID: 10917659
Add a reference
References: 8 entries
Toska et al. 2014. Oncogene. 33(43):5100-8. PMID: 24166496.
Prohibitin is required for transcriptional repression by the WT1-BASP1 complex.
Ozkucur et al. 2011. BMC Cell Biol. 12:4. PMID: 21255452.
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
Osborne et al. 2001. J Cell Sci. 114(Pt 13):2501-11. PMID: 11559758.
Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing.
Thomas et al. 1999. Biochem Soc Trans. 27(4):648-52. PMID: 10917659.
Generation of phosphatidylinositol-specific antibodies and their characterization.
Add a reference
