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Anti-PIP2 [PIP2 2C11]


Catalogue Number 151237
Applications ChIP ELISA IHC IF IP Fn WB
Antigen/Gene or Protein Targets Phosphatidylinositol biphosphate (PIP2)
Reactivity N/A
Relevance PtdIns 4,5-P2 is a membrane phospholipid and an important component of signal transduction pathways.
Host Mouse
Immunogen Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.
Subclass IgM
Positive Control To test the Ab efficiency use a lipid blot assay as described in References above.
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes It is recommended to use a lipid blot assay or nuclear staining to test this antibody. Cross reacts with PtdIns(4,5)P2, Ptd(4)P and Ptd(3,4,5)P3.
Research Area Cell Signaling & Signal Transduction, Neurobiology

References: 4 entries

Toska et al. 2014. Oncogene. 33(43):5100-8. PMID: 24166496.

Ozkucur et al. 2011. BMC Cell Biol. 12:4. PMID: 21255452.

Osborne et al. 2001. J Cell Sci. 114(Pt 13):2501-11. PMID: 11559758.

Thomas et al. 1999. Biochem Soc Trans. 27(4):648-52. PMID: 10917659.

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