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Anti-HLADR ß chain 1 [DA2]
RECENTLY UPDATED

Invented by Prof Walter Bodmer from University of Oxford
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields

Info

Catalogue Number 151225
Applications ELISA FACS IHC WB
Antigen/Gene or Protein Targets Human Leukocyte Antigen DR ß chain 1 (1st domain, monomorphic, MHC class II)
Reactivity Human
Relevance Monoclonal antibody which binds all DR and DP antigens, with use in tissue and HLA typing.

Background and Research Application
Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.
DA2 may be used for tissue typing. This antibody will bind all DR and DP antigens except DR7, and can aid with HLA typing.
Host Mouse
Immunogen The membranes of LKT cells (a B lymphoblastoid cell line)
Immunogen UniProt ID P01911
Subclass IgG1
Myeloma Used P3X63Ag8
Recommended Growing Conditions RPMI 1640 + 10% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
DA2 will detect dimers and the beta chain by western blot. DA2 will bind all DR and DP antigens except DR7.

Concentration
1mg/ml as standard
Research Area Immunology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Brodsky et al. 1980. Tissue Antigens. 16(1):30-48. PMID: 6162228.

Monoclonal antibodies to HLA--DRw determinants.

Europe PMC ID: 6162228

Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.

Monoclonal antibodies for analysis of the HLA system.

Europe PMC ID: 95015


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References: 4 entries

Brodsky et al. 1980. Tissue Antigens. 16(1):30-48. PMID: 6162228.

Monoclonal antibodies to HLA--DRw determinants.

Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.

Monoclonal antibodies for analysis of the HLA system.


Add a reference