Anti-HLADR ß chain 1 [DA2]
Invented by Prof Walter Bodmer from University of Oxford
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151225 |
| Applications | ELISA FACS IHC WB |
| Antigen/Gene or Protein Targets | Human Leukocyte Antigen DR ß chain 1 (1st domain, monomorphic, MHC class II) |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which binds all DR and DP antigens, with use in tissue and HLA typing. Background and Research Application Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response. DA2 may be used for tissue typing. This antibody will bind all DR and DP antigens except DR7, and can aid with HLA typing. |
| Host | Mouse |
| Immunogen | The membranes of LKT cells (a B lymphoblastoid cell line) |
| Immunogen UniProt ID | P01911 |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest DA2 will detect dimers and the beta chain by western blot. DA2 will bind all DR and DP antigens except DR7. Concentration 1mg/ml as standard |
| Research Area | Immunology |
References: 4 entries
Brodsky et al. 1980. Tissue Antigens. 16(1):30-48. PMID: 6162228.
Monoclonal antibodies to HLA--DRw determinants.
Europe PMC ID: 6162228
Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
Monoclonal antibodies for analysis of the HLA system.
Europe PMC ID: 95015
Add a reference
References: 4 entries
Brodsky et al. 1980. Tissue Antigens. 16(1):30-48. PMID: 6162228.
Monoclonal antibodies to HLA--DRw determinants.
Brodsky et al. 1979. Immunol Rev. 47:3-61. PMID: 95015.
Monoclonal antibodies for analysis of the HLA system.
Add a reference
