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Monoclonal Antibodies

Anti-SIGLEC3 [CD33 3D6]

Invented by Prof Paul Crocker from University of Dundee
Invented at University of Oxford
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Adapted from Taylor VC, Buckley CD, Douglas M, Cody AJ, Simmons DL, Freeman SD. The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2. J Biol Chem. 1999 Apr 23;274(17):11505-12. doi: 10.1074/jbc.274.17.11505. PMID: 10206955.Tyrosine phosphorylation of CD33 after pervanadate treatment. U937 and THP-1 cells were either treated with 1 mM pervanadate for 15 min at 37 °C (+) or left unstimulated (−). Lysates were precipitated (IP) either with mouse IgG1 control (mIgG1) or with anti-CD33, 1C7/1 (CD33), both antibodies precoupled to protein G-Sepharose. The immunoprecipitated proteins were separated by SDS-PAGE and analyzed by immunoblotting with anti-phosphotyrosine (PY20) or anti-CD33 (1C7/1+3D6/1).
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Catalogue Number 151219
Applications ELISA
Antigen/Gene or Protein Targets SIGLEC3 (CD33)
Synonyms Sialic Acid Binding Ig Like Lectin 12; SIGLECL1; SLG 3; Siglec-12; Siglec-L1; S2V
Reactivity Human
Relevance The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
Host Mouse
Immunogen CD33-Fc recombinant protein
Positive Control CD33 transfected COS cells
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Cancer, Cell Type or Organelle Marker, Immunology, Stem Cell Biology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Taylor et al. 1999. J Biol Chem. 274(17):11505-12. PMID: 10206955.

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The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2.

Europe PMC ID: 10206955

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Freeman et al. 1995. Blood. 85(8):2005-12. PMID: 7718872.

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Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules.

Europe PMC ID: 7718872

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References: 4 entries

Taylor et al. 1999. J Biol Chem. 274(17):11505-12. PMID: 10206955.

View  

The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2.

View  

Freeman et al. 1995. Blood. 85(8):2005-12. PMID: 7718872.

View  

Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules.

View  

Add a reference

Inventor Information