Anti-MUC18 [OJ79c MUC 18]
Invented by Helen Turley from University of Oxford
Invented at University of Oxford
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- References (12)
- Inventor Info
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![Adapted from Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, Greenman J, Boyle RW. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 2011 Feb;132(2):256-65. doi: 10.1111/j.1365-2567.2010.03359.x. Epub 2010 Oct 29. PMID: 21039468; PMCID: PMC3045661.Conjugated and unconjugated anti-CD326, anti-CD146 and anti-CD104 were analysed by flow cytometry for binding to CORL23 and LoVo cells [black line, negative control; blue line, unconjugated antibody; red line, immunoconjugates with porphyrin 1 (a) or porphyrin 2 (b)].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/855cef97-9b46-4cfb-b835-3da91be75565.jpg)
![Adapted from Pruszak J, Sonntag KC, Aung MH, Sanchez-Pernaute R, Isacson O. Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. Stem Cells. 2007 Sep;25(9):2257-68. doi: 10.1634/stemcells.2006-0744. Epub 2007 Jun 21. PMID: 17588935; PMCID: PMC2238728. Surface antigens detected during neural differentiation of human embryonic stem cells (hESC). (A): Expression of embryonic stem cell markers such as SSEA-4 and Tra-1-60 is downregulated upon neural differentiation of hESC in vitro. Nonetheless, nests of immature hESC, here a cell cluster labeled with the surface antigen Tra-1-81, remain present after neural induction. (B): Presence of the CD133 antigen as a somatic stem cell marker was found to be localized on clusters of proliferative neuroepithelial cells, most prominently on the apical side of the neural precursors, toward the lumen (*) of the neural rosette structures (dotted line). Scale bar: 20 μm. (C): Early intermediate surface markers such as SSEA-1 are not present on immature hESC (in contrast to mouse embryonic stem cells, which are SSEA-1+ [34, 42]). During neural induction, presence of SSEA-1 emerges on Sox1+ neuroepithelial rosette cells, which may allow for positive selection at the earlier stage of the differentiation protocol. More differentiated process-bearing DCX-positive cells are SSEA-1 negative. Scale bars: 50 μm; far right panel: 20 μm. (D–H): Fluorescence-activated cell sorting (FACS) profile of neural surface markers present during neuronal differentiation of hESC at the late neural precursor stage (div 30–37). Flow cytometric analysis and corresponding immunocytochemistry for the p75 (CD271), A2B5, CD24, and CD29 surface antigens are shown. (I): Combinatorial FACS analysis for presumptive neural stem cell markers: a subset of CD29+ and SSEA-1+ cells coexpresses the CD133 antigen. (J): Cells positive for the CD146 antigen do not coexpress FORSE-1, suggesting the presence of distinct neural precursor cell populations. (K, L): Such neural markers could be used to select against unwanted immature stem cells: CD146+ and NCAM+ cell populations do not coexpress the immature hESC markers SSEA-3 or Tra-1-61. Scale bars: 50 μm. FACS plots: black line, control; green line, stained sample. Abbreviations: DCX, doublecortin; div, days in vitro; FORSE1, forebrain surface embryonic antigen-1; max., maximum; NCAM, neural cell adhesion molecule; SSEA, stage-specific embryonic antigen; TuJ1, β-III-tubulin.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/b525014b-4681-47ae-8dfa-6d37ee99ab04.jpg)
![Adapted from Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, Greenman J, Boyle RW. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 2011 Feb;132(2):256-65. doi: 10.1111/j.1365-2567.2010.03359.x. Epub 2010 Oct 29. PMID: 21039468; PMCID: PMC3045661.Conjugated and unconjugated anti-CD326, anti-CD146 and anti-CD104 were analysed by flow cytometry for binding to CORL23 and LoVo cells [black line, negative control; blue line, unconjugated antibody; red line, immunoconjugates with porphyrin 1 (a) or porphyrin 2 (b)].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/855cef97-9b46-4cfb-b835-3da91be75565.jpg)
![Adapted from Pruszak J, Sonntag KC, Aung MH, Sanchez-Pernaute R, Isacson O. Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. Stem Cells. 2007 Sep;25(9):2257-68. doi: 10.1634/stemcells.2006-0744. Epub 2007 Jun 21. PMID: 17588935; PMCID: PMC2238728. Surface antigens detected during neural differentiation of human embryonic stem cells (hESC). (A): Expression of embryonic stem cell markers such as SSEA-4 and Tra-1-60 is downregulated upon neural differentiation of hESC in vitro. Nonetheless, nests of immature hESC, here a cell cluster labeled with the surface antigen Tra-1-81, remain present after neural induction. (B): Presence of the CD133 antigen as a somatic stem cell marker was found to be localized on clusters of proliferative neuroepithelial cells, most prominently on the apical side of the neural precursors, toward the lumen (*) of the neural rosette structures (dotted line). Scale bar: 20 μm. (C): Early intermediate surface markers such as SSEA-1 are not present on immature hESC (in contrast to mouse embryonic stem cells, which are SSEA-1+ [34, 42]). During neural induction, presence of SSEA-1 emerges on Sox1+ neuroepithelial rosette cells, which may allow for positive selection at the earlier stage of the differentiation protocol. More differentiated process-bearing DCX-positive cells are SSEA-1 negative. Scale bars: 50 μm; far right panel: 20 μm. (D–H): Fluorescence-activated cell sorting (FACS) profile of neural surface markers present during neuronal differentiation of hESC at the late neural precursor stage (div 30–37). Flow cytometric analysis and corresponding immunocytochemistry for the p75 (CD271), A2B5, CD24, and CD29 surface antigens are shown. (I): Combinatorial FACS analysis for presumptive neural stem cell markers: a subset of CD29+ and SSEA-1+ cells coexpresses the CD133 antigen. (J): Cells positive for the CD146 antigen do not coexpress FORSE-1, suggesting the presence of distinct neural precursor cell populations. (K, L): Such neural markers could be used to select against unwanted immature stem cells: CD146+ and NCAM+ cell populations do not coexpress the immature hESC markers SSEA-3 or Tra-1-61. Scale bars: 50 μm. FACS plots: black line, control; green line, stained sample. Abbreviations: DCX, doublecortin; div, days in vitro; FORSE1, forebrain surface embryonic antigen-1; max., maximum; NCAM, neural cell adhesion molecule; SSEA, stage-specific embryonic antigen; TuJ1, β-III-tubulin.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/b525014b-4681-47ae-8dfa-6d37ee99ab04.jpg)
![Image thumbnail for Anti-MUC18 [OJ79c MUC 18]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/855cef97-9b46-4cfb-b835-3da91be75565.jpg)
![Image thumbnail for Anti-MUC18 [OJ79c MUC 18]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/34d40549-5d10-4c38-b45a-f63a98b511b5.jpg)
![Image thumbnail for Anti-MUC18 [OJ79c MUC 18]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/cd497553-9302-4f37-9cf8-c0485c7e047e.jpg)
![Image thumbnail for Anti-MUC18 [OJ79c MUC 18]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/b525014b-4681-47ae-8dfa-6d37ee99ab04.jpg)
| Catalogue Number | 151214 |
| Applications | FACS IHC IF |
| Antigen/Gene or Protein Targets | MUC18 (CD146) |
| Synonyms | ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against a marker of melanoma and metastasis. Background and Research Application CD146 has been identified as a cell adhesion molecule and is useful in differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 is expressed at cell-cell junctions in all endothelial cells. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogenous tumour spread. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease. It is associated with poor prognosis. |
| Host | Mouse |
| Immunogen | Recombinant MUC18 (D1-D5) Fc chimeric protein |
| Immunogen UniProt ID | P43121 |
| Positive Control | A-375 cell, SK-MEL-24 cell, HUV-EC or HeLa cell lysates. Tonsil or Melanoma. |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 130kDa |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | RPMI + 10% FCS + HAT |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest CD146 is expressed only sporadically in benign melanomas that have a low probability of metastasizing. However, with increasing tumour thickness, CD146 expression becomes more frequent and it is found on a large proportion of advanced primary tumours and metastases. Cellular Localization: Cell surface Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1.0ug/ml); Concentration 1mg/ml as standard |
| Research Area | Adhesion, Cancer, Cardiovascular, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology |
References: 12 entries
Smith et al. 2011. Immunology. 132(2):256-65. PMID: 21039468.
Sato et al. 2010. Mod Pathol. 23(11):1458-66. PMID: 20657552.
Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
Europe PMC ID: 21039468
Iohara et al. 2008. Stem Cells. 26(9):2408-18. PMID: 18583536.
Crisan et al. 2008. Cell Stem Cell. 3(3):301-13. PMID: 18786417.
A perivascular origin for mesenchymal stem cells in multiple human organs.
Europe PMC ID: 18786417
A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
Europe PMC ID: 18583536
Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.
Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.
Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.
Europe PMC ID: 17588935
Kuzu et al. 1993. Lab Invest. 69(3):322-8. PMID: 7690867.
Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.
Europe PMC ID: 7690867
Add a reference
References: 12 entries
Smith et al. 2011. Immunology. 132(2):256-65. PMID: 21039468.
Sato et al. 2010. Mod Pathol. 23(11):1458-66. PMID: 20657552.
Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
Iohara et al. 2008. Stem Cells. 26(9):2408-18. PMID: 18583536.
Crisan et al. 2008. Cell Stem Cell. 3(3):301-13. PMID: 18786417.
A perivascular origin for mesenchymal stem cells in multiple human organs.
A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.
Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.
Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.
Kuzu et al. 1993. Lab Invest. 69(3):322-8. PMID: 7690867.
Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.
Add a reference
