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Anti-MUC18 [OJ79c MUC 18]

Invented by Helen Turley at University of Oxford

Info

Catalogue Number 151214
Applications FACS IHC IF
Antigen/Gene or Protein Targets MUC18 (CD146)
Synonyms ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227
Reactivity Human
Relevance Monoclonal antibody directed against a marker of melanoma and metastasis.

Background and Research Application
CD146 has been identified as a cell adhesion molecule and is useful in differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 is expressed at cell-cell junctions in all endothelial cells. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogenous tumour spread.
It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease. It is associated with poor prognosis.
Host Mouse
Immunogen Recombinant MUC18 (D1-D5) Fc chimeric protein
Immunogen UniProt ID P43121
Positive Control A-375 cell, SK-MEL-24 cell, HUV-EC or HeLa cell lysates. Tonsil or Melanoma.
Subclass IgG1 kappa
Molecular Weight (kDa) 130kDa
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions RPMI + 10% FCS + HAT
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
CD146 is expressed only sporadically in benign melanomas that have a low probability of metastasizing. However, with increasing tumour thickness, CD146 expression becomes more frequent and it is found on a large proportion of advanced primary tumours and metastases.

Cellular Localization: Cell surface
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1.0ug/ml);

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer, Cardiovascular, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References

There are 11 reference entries for this reagent.

View All References

References: 11 entries

Smith et al. 2011. Immunology. 132(2):256-65. PMID: 21039468.

Sato et al. 2010. Mod Pathol. 23(11):1458-66. PMID: 20657552.

Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.

Europe PMC ID: 21039468

Iohara et al. 2008. Stem Cells. 26(9):2408-18. PMID: 18583536.

Crisan et al. 2008. Cell Stem Cell. 3(3):301-13. PMID: 18786417.

A perivascular origin for mesenchymal stem cells in multiple human organs.

Europe PMC ID: 18786417

A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.

Europe PMC ID: 18583536

Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.

Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.

Europe PMC ID: 17588935

Kuzu et al. 1993. Lab Invest. 69(3):322-8. PMID: 7690867.

Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.

Europe PMC ID: 7690867


Add a reference

References: 11 entries

Smith et al. 2011. Immunology. 132(2):256-65. PMID: 21039468.

Sato et al. 2010. Mod Pathol. 23(11):1458-66. PMID: 20657552.

Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.

Iohara et al. 2008. Stem Cells. 26(9):2408-18. PMID: 18583536.

Crisan et al. 2008. Cell Stem Cell. 3(3):301-13. PMID: 18786417.

A perivascular origin for mesenchymal stem cells in multiple human organs.

A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.

Pruszak et al. 2007. Stem Cells. 25(9):2257-68. PMID: 17588935.

Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.

Kuzu et al. 1993. Lab Invest. 69(3):322-8. PMID: 7690867.

Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors.


Add a reference