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Monoclonal Antibodies

Anti-ATF1 [ATF1 2A9/8]

Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Gómez-Gil et al. 2020. Elife. 9. PMID: 32915139. Figure. S. pombe wild type cells expressing a genomic Sty1-HA6his fusion were grown in YES medium to mid-log phase, and remained untreated (0), or treated with a range of concentrations of LatA for 1 hr. Activated/total Sty1 were detected with anti-phospho-p38 and anti-HA antibodies, respectively. Total Atf1 levels were detected with anti-Atf1 antibody. Anti-Cdc2 was used as a loading control.
Adapted from Prieto-Ruiz et al. 2020. mBio; 11(1). PMID: 31911490. Figure. Total extracts from growing cultures of wild-type and rnc1Δ strains, treated with 0.6 M KCl for the indicated times, were resolved by SDS-PAGE, and the Atf1 protein levels were detected by incubation with anti-Atf1. Anti-Cdc2 was used as a loading control. *, P < 0.05.
Adapted from Prieto-Ruiz et al. 2020. mBio; 11(1). PMID: 31911490. Figure. Total extracts from growing cultures of Rnc1-3HA (wild-type), Rnc1(mKH)-3HA, and Rnc1(S/T6A)-3HA cells growing exponentially and were treated with 0.6 M KCl for the indicated times. Total levels of Atf1 were detected by incubation with anti-Atf1 antibody. Anti-Cdc2 was used as a loading control.
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Catalogue Number 151204
Applications ChIP IHC IP WB
Antigen/Gene or Protein Targets Activating Transcription Factor 1 (ATF1)
Reactivity Human
Relevance The atf1 gene of S. pombe encodes a bZIP transription factor with strong homology to the mammalian factor ATF2. This is regulated by the stress activated mitogen-activated protein kinases SAPK/JNK and p38.
Host Mouse
Immunogen Yeast transcription factor ATF1
Subclass IgG2a
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Epigenetics & Nuclear Signalling

References

There are 5 reference entries for this reagent.

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References: 5 entries

Gómez-Gil et al. 2020. Elife. 9:. PMID: 32915139.

WB

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Prieto-Ruiz et al. 2020. mBio. 11(1):. PMID: 31911490.

WB

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Asada et al. 2017. Nucleic Acids Res. 45(16):9361-9371. PMID: 28934464.

ChIP

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Wilkinson et al. 1996. Genes Dev. 10(18):2289-301. PMID: 8824588.

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The Atf1 transcription factor is a target for the Sty1 stress-activated MAP kinase pathway in fission yeast.

Europe PMC ID: 8824588

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References: 5 entries

Gómez-Gil et al. 2020. Elife. 9:. PMID: 32915139.

WB

View  

Prieto-Ruiz et al. 2020. mBio. 11(1):. PMID: 31911490.

WB

View  

Asada et al. 2017. Nucleic Acids Res. 45(16):9361-9371. PMID: 28934464.

ChIP

View  

Wilkinson et al. 1996. Genes Dev. 10(18):2289-301. PMID: 8824588.

View  

The Atf1 transcription factor is a target for the Sty1 stress-activated MAP kinase pathway in fission yeast.

View  

Add a reference

Inventor Information