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Monoclonal Antibodies

Anti-Cdk1 [Y100.4]

Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Adapted from Chang et al. 2013. PLoS Genet. 9(11):e1003936. PMID: 24244195 Figure. Epistasis analysis of poz1Δ, rap1Δ, and taz1Δ cells. (A) Schematic model of telomere maintenance regulation in fission yeast. (B) Quantification of telomere length distribution for Southern blot analysis shown in Figure S1A. (C, D) Recruitment of Rap1 (C) and Trt1TERT (D) to telomeres, monitored by dot blot-based ChIP assays. Compared to wt cells, Rap1 showed statistically significant reductions in binding to telomeres in taz1Δ (p = 2.5×10−3) and poz1Δ taz1Δ (p = 2.6×10−5) cells. Rap1 still showed statistically significant binding to telomeres even in poz1Δ taz1Δ cells compared to no tag control strain (p = 1.4×10−5). Error bars correspond to the standard error of the mean (SEM) from multiple independent experiments. Anti-myc western blot analysis indicated comparable expression levels in different genetic backgrounds. Cdc2 western blot served as a loading control.
Adapted from Mennie et al. 2018. Nat Commun. 9(1):586. PMID: 29422503. Figure. Expression levels of (d) Pof8 and (e) Trt1 used in ChIP assays were monitored by western blot analysis. Anti-Cdc2 blots served as loading control. Molecular weight (kDa) of size markers are also indicated
Adapted from Radcliffe et al. 1998. Mol Biol Cell. 9(7):1757-71. PMID: 9658169. Figure. ts nda3-1828 (DH12) Cell extracts (20 μg) were run in SDS-PAGE, and immunoblotting was performed with mouse anti-β-tubulin antibody (Sigma) (top) or anti-Cdc2 antibody as a loading control (bottom)
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Catalogue Number 151185
Applications WB
Antigen/Gene or Protein Targets Cyclin Dependent Kinase 1 (Cdk1, Cdc2)
Synonyms Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; CDC2; Cell Cycle Controller CDC2; CDKN1
Reactivity Schizosaccharomyces pombe
Relevance Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.
Host Mouse
Immunogen Peptide spanning the C-terminal 14 residues of S. pombe Cdk1.
Subclass IgG2b
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Cancer, Cell Cycle, Cell Type or Organelle Marker, Neurobiology

References

There are 7 reference entries for this reagent.

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References: 7 entries

Mennie et al. 2018. Nat Commun. 9(1):586. PMID: 29422503.

WB

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Chang et al. 2013. PLoS Genet. 9(11):e1003936. PMID: 24244195.

WB

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Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.

Europe PMC ID: 24244195

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Radcliffe et al. 1998. Mol Biol Cell. 9(7):1757-71. PMID: 9658169.

WB

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Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.

Europe PMC ID: 9658169

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Yamano et al. 1996. EMBO J. 15(19):5268-79. PMID: 8895572.

WB

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The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe.

Europe PMC ID: 8895572

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Add a reference

References: 7 entries

Mennie et al. 2018. Nat Commun. 9(1):586. PMID: 29422503.

WB

View  

Chang et al. 2013. PLoS Genet. 9(11):e1003936. PMID: 24244195.

WB

View  

Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.

View  

Radcliffe et al. 1998. Mol Biol Cell. 9(7):1757-71. PMID: 9658169.

WB

View  

Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.

View  

Yamano et al. 1996. EMBO J. 15(19):5268-79. PMID: 8895572.

WB

View  

The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe.

View  

Add a reference

Inventor Information