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Anti-L1CAM [UJ127.11]

Invented by Dr JOHN KEMSHEAD at SHIRE PHARMACEUTICALS

Info

Catalogue Number 151178
Applications ELISA FACS IHC IF IP WB
Antigen/Gene or Protein Targets L1 cell adhesion molecule (L1CAM; CD171)
Synonyms CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1
Reactivity Human and Mouse
Relevance L1 cell adhesion molecule (L1CAM) is a cell-surface glycoprotein of 220-240kDa (doublet) and member of the neural adhesion molecule family of the immunoglobulin superfamily. L1CAM is implicated in the development of the nervous system and its expression is restricted to tissues arising from the neuroectoderm. L1CAM is present on tumours of neuroectodermal and glial origin (e.g. neuroblastoma and schwannomas). UJ127.11 may be useful in the diagnosis of embryonic tumours (e.g. neuroblastoma) or for the purpose of bone marrow purging. The antigen is present on tumours of neuroectodermal and glial origin.eg neuroblastoma and schwannomas.
Host Mouse
Immunogen Homogenous suspension of 16 week human foetal brain.
Subclass IgG1 kappa
Positive Control Frozen mouse E16.5 lung sections (IHC), Cell lines TR 14, LAN-1, CHP 100, CHP212, CHP126 ( Neuroblastomas or Schwannomas) (IF), M5 melanoma cells or human foetal brain (WB)
Molecular Weight (kDa) 220-240kDa
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Cellular Localization: Cell surface
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml);
Research Area Adhesion, Cell Signaling & Signal Transduction, Neurobiology

References: 12 entries

Lund et al. 2015. PLoS One. 10(4):e0123684. PMID: 25860483.

IF

Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment.

Europe PMC ID: 25860483

Matsuda et al. 2013. J Proteomics. 85:1-11. PMID: 23612463.

ELISA IP WB

Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma.

Europe PMC ID: 23612463

Morales et al. 2012. Mol Cell Proteomics. 11(6):M111.011973. PMID: 22186713.

Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.

Europe PMC ID: 22186713

Heiz et al. 2004. J Biol Chem. 279(30):31149-56. PMID: 15151998.

Hepatocyte growth factor-induced ectodomain shedding of cell adhesion molecule L1: role of the L1 cytoplasmic domain.

Europe PMC ID: 15151998

Patel et al. 1990. Biochem Soc Trans. 18(2):274. PMID: 2379713.

Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule.

Europe PMC ID: 2379713

Kemshead et al. 1983. Int J Cancer. 31(2):187-95. PMID: 6826247.

Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells.

Europe PMC ID: 6826247


Add a reference

References: 12 entries

Lund et al. 2015. PLoS One. 10(4):e0123684. PMID: 25860483.

IF

Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment.

Europe PMC ID: 25860483

Matsuda et al. 2013. J Proteomics. 85:1-11. PMID: 23612463.

ELISA IP WB

Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma.

Europe PMC ID: 23612463

Morales et al. 2012. Mol Cell Proteomics. 11(6):M111.011973. PMID: 22186713.

Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.

Europe PMC ID: 22186713

Heiz et al. 2004. J Biol Chem. 279(30):31149-56. PMID: 15151998.

Hepatocyte growth factor-induced ectodomain shedding of cell adhesion molecule L1: role of the L1 cytoplasmic domain.

Europe PMC ID: 15151998

Patel et al. 1990. Biochem Soc Trans. 18(2):274. PMID: 2379713.

Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule.

Europe PMC ID: 2379713

Kemshead et al. 1983. Int J Cancer. 31(2):187-95. PMID: 6826247.

Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells.

Europe PMC ID: 6826247


Add a reference

References: 12 entries

Lund et al. 2015. PLoS One. 10(4):e0123684. PMID: 25860483.

IF

Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment.

Matsuda et al. 2013. J Proteomics. 85:1-11. PMID: 23612463.

ELISA IP WB

Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma.

Morales et al. 2012. Mol Cell Proteomics. 11(6):M111.011973. PMID: 22186713.

Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.

Heiz et al. 2004. J Biol Chem. 279(30):31149-56. PMID: 15151998.

Hepatocyte growth factor-induced ectodomain shedding of cell adhesion molecule L1: role of the L1 cytoplasmic domain.

Patel et al. 1990. Biochem Soc Trans. 18(2):274. PMID: 2379713.

Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule.

Kemshead et al. 1983. Int J Cancer. 31(2):187-95. PMID: 6826247.

Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells.


Add a reference


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