Cancer Research Technology
Log in Register
Menu

Anti-L1CAM [UJ127.11]

Invented by Dr JOHN KEMSHEAD at Institute Of Child Health

Info

Catalogue Number 151178
Applications ELISA FACS IHC IF IP WB
Antigen/Gene or Protein Targets L1 cell adhesion molecule (L1CAM; CD171)
Synonyms CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1
Reactivity Human and Mouse
Relevance Monoclonal antibody capable of differentiating between brain tumours of neural origin rather than glial origin.

Background and Research Application
Anti-L1CAM (UJ127.11) recognises L1 cell adhesion molecule (L1CAM), a cell-surface glycoprotein of 220-240kDa (doublet) and member of the neural adhesion molecule family of the immunoglobulin superfamily. UJ127.11 may be useful in the diagnosis of embryonic tumours (e.g. neuroblastoma) or for the purpose of bone marrow purging. Studies on normal foetal and adult tissues show that UJ127:11 recognises antigen restricted in its expression to cells of neural rather than glial origin. Neural tumours such as neuroblastoma, medulloblastoma, schwannomas and ganglioglioma bind the monoclonal antibody whereas malignancies originating from glial cells do not bind UJ 127:11 – this allows for diagnosis of the origin of brain tumour. This antibody has little cross-reactivity with tissues outside of the neuroectoderm.

L1CAM is implicated in the development of the nervous system and its expression is restricted to tissues arising from the neuroectoderm. L1CAM is present on tumours of neuroectodermal and glial origin (e.g. neuroblastoma and schwannomas). L1CAM also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.
Host Mouse
Immunogen Homogenous suspension of 16 week human foetal brain.
Immunogen UniProt ID P32004
Positive Control Frozen mouse E16.5 lung sections (IHC), Cell lines TR 14, LAN-1, CHP 100, CHP212, CHP126 ( Neuroblastomas or Schwannomas) (IF), M5 melanoma cells or human foetal brain (WB)
Subclass IgG1 kappa
Molecular Weight (kDa) 220-240kDa
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
UJ 127:11 has proved useful in the diagnosis of metastatic spread of neuroblastoma to bone marrow, as the antibody does not react with normal haemopoietic cells from foetal, paediatric or adult bone marrow. Anti-L1CAM recognises antigen in both reducing and non-reducing conditions. This antibody was made via avoiding immunizing mice with human neuroblastoma tumour, which is often calcified and necrotic, and instead chose fresh human foetal brain as an immunogen, as many reports have described the expression of foetal antigens on human tumour cells.

Frozen sections of adult cerebellum, temporal lobe, adrenal medulla and nerve plexi in the colon and oesophagus were found to contain cells expressing the UJ127:11 antigen, thus anti-UJ127:11 only binds to tissue of neuroectodermal origin. This antibody does not bind to any pre-B, B, T, on-B non-T, promyelocytic or myeloid leukemic cell line.

Cellular Localization: Cell surface
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml);

Concentration
1mg/ml as standard
Research Area Adhesion, Cell Signaling & Signal Transduction, Neurobiology

References

There are 17 reference entries for this reagent.

View All References

References: 17 entries

Maten et al. 2019. Int J Mol Sci. 20(17):. PMID: 31455004.

Pace et al. 2019. Int J Mol Sci. 20(16):. PMID: 31426278.

Stevers et al. 2019. Mod Pathol. 32(1):88-99. PMID: 30171198.

Er et al. 2018. Nat Cell Biol. 20(8):966-978. PMID: 30038252.

Wood et al. 2018. Brain Pathol. 28(2):192-202. PMID: 28960623.

Lund et al. 2015. PLoS One. 10(4):e0123684. PMID: 25860483.

IF

Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment.

Europe PMC ID: 25860483

Matsuda et al. 2013. J Proteomics. 85:1-11. PMID: 23612463.

ELISA IP WB

Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma.

Europe PMC ID: 23612463

Morales et al. 2012. Mol Cell Proteomics. 11(6):M111.011973. PMID: 22186713.

Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.

Europe PMC ID: 22186713

Heiz et al. 2004. J Biol Chem. 279(30):31149-56. PMID: 15151998.

Hepatocyte growth factor-induced ectodomain shedding of cell adhesion molecule L1: role of the L1 cytoplasmic domain.

Europe PMC ID: 15151998

Patel et al. 1990. Biochem Soc Trans. 18(2):274. PMID: 2379713.

Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule.

Europe PMC ID: 2379713

Kemshead et al. 1983. Int J Cancer. 31(2):187-95. PMID: 6826247.

Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells.

Europe PMC ID: 6826247


Add a reference

References: 17 entries

Maten et al. 2019. Int J Mol Sci. 20(17):. PMID: 31455004.

Pace et al. 2019. Int J Mol Sci. 20(16):. PMID: 31426278.

Stevers et al. 2019. Mod Pathol. 32(1):88-99. PMID: 30171198.

Er et al. 2018. Nat Cell Biol. 20(8):966-978. PMID: 30038252.

Wood et al. 2018. Brain Pathol. 28(2):192-202. PMID: 28960623.

Lund et al. 2015. PLoS One. 10(4):e0123684. PMID: 25860483.

IF

Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment.

Matsuda et al. 2013. J Proteomics. 85:1-11. PMID: 23612463.

ELISA IP WB

Glycoproteomics-based cancer marker discovery adopting dual enrichment with Wisteria floribunda agglutinin for high specific glyco-diagnosis of cholangiocarcinoma.

Morales et al. 2012. Mol Cell Proteomics. 11(6):M111.011973. PMID: 22186713.

Alterations in protein regulators of neurodevelopment in the cerebrospinal fluid of infants with posthemorrhagic hydrocephalus of prematurity.

Heiz et al. 2004. J Biol Chem. 279(30):31149-56. PMID: 15151998.

Hepatocyte growth factor-induced ectodomain shedding of cell adhesion molecule L1: role of the L1 cytoplasmic domain.

Patel et al. 1990. Biochem Soc Trans. 18(2):274. PMID: 2379713.

Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule.

Kemshead et al. 1983. Int J Cancer. 31(2):187-95. PMID: 6826247.

Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells.


Add a reference