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Anti-L1CAM [UJ127.11]

Info

Catalogue Number 151178
Applications ELISA FACS IHC IF IP WB
Antigen/Gene or Protein Targets L1 cell adhesion molecule (L1CAM; CD171)
Synonyms CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1
Reactivity Human and Mouse
Relevance L1 cell adhesion molecule (L1CAM) is a cell-surface glycoprotein of 220-240kDa (doublet) and member of the neural adhesion molecule family of the immunoglobulin superfamily. L1CAM is implicated in the development of the nervous system and its expression is restricted to tissues arising from the neuroectoderm. L1CAM is present on tumours of neuroectodermal and glial origin (e.g. neuroblastoma and schwannomas). UJ127.11 may be useful in the diagnosis of embryonic tumours (e.g. neuroblastoma) or for the purpose of bone marrow purging. The antigen is present on tumours of neuroectodermal and glial origin.eg neuroblastoma and schwannomas.
Host Mouse
Immunogen Homogenous suspension of 16 week human foetal brain.
Subclass IgG1 kappa
Positive Control Frozen mouse E16.5 lung sections (IHC), Cell lines TR 14, LAN-1, CHP 100, CHP212, CHP126 ( Neuroblastomas or Schwannomas) (IF), M5 melanoma cells or human foetal brain (WB)
Molecular Weight (kDa) 220-240kDa
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Cellular Localization: Cell surface
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml);
Research Area Adhesion, Cell Signaling & Signal Transduction, Neurobiology

References: 6 entries

Lund et al. 2015. PLoS One. 10(4):e0123684. PMID: 25860483.

IF

Matsuda et al. 2013. J Proteomics. 85:1-11. PMID: 23612463.

ELISA IP WB

Morales et al. 2012. Mol Cell Proteomics. 11(6):M111.011973. PMID: 22186713.

Heiz et al. 2004. J Biol Chem. 279(30):31149-56. PMID: 15151998.

Patel et al. 1990. Biochem Soc Trans. 18(2):274. PMID: 2379713.

Kemshead et al. 1983. Int J Cancer. 31(2):187-95. PMID: 6826247.


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