Cancer Research Technology
Log in Register
Menu

Anti-CD45RO [UCH-L1]

Invented by Prof Peter Beverley at Imperial College

Info

Catalogue Number 151173
Applications FACS IHC IP WB
Antigen/Gene or Protein Targets Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45RO)
Synonyms Protein Tyrosine Phosphatase; Receptor Type C; CD45 Antigen; CD45; L-CA; T200; Protein Tyrosine Phosphatase; Receptor Type; C Polypeptide; T200 Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; GP180; CD45R; B220; LCA; LY5
Reactivity Human
Relevance PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). CD45R and CD45RO are two of multiple PTPRC isoforms generated by alternative splicing. CD45R is found on B lymphocyte and T lymphocyte subsets. CD45RO is present on 50% of T cells, most granulocytes and monocytes and most mature T cell tumours. This antibody reacts with an epitope unique for CD45R0. It may identify abnormalities of functional T-cell subsets in immune disorders, be used in tumour diagnosis by FACS or immunohistology and has been very useful in studies of human T cell function and ontogeny.
Host Mouse
Immunogen Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
Subclass IgG2a
Molecular Weight (kDa) 180
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS.
Strain Balb/c
Research Area Cancer, Cell Type or Organelle Marker, Epitope Tags, Immunology

References: 10 entries

Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766.

Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.

Europe PMC ID: 23627766

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

Europe PMC ID: 11694532

Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028.

CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO.

Europe PMC ID: 11593028

Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210.

Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis.

Europe PMC ID: 11422210

Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439.

Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

Europe PMC ID: 2423439


Add a reference

References: 10 entries

Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766.

Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.

Europe PMC ID: 23627766

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

Europe PMC ID: 11694532

Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028.

CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO.

Europe PMC ID: 11593028

Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210.

Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis.

Europe PMC ID: 11422210

Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439.

Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

Europe PMC ID: 2423439


Add a reference

References: 10 entries

Zlobec et al. 2013. J Transl Med. 11:104. PMID: 23627766.

Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types.

Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.

Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.

Ishii et al. 2001. Proc Natl Acad Sci U S A. 98(21):12138-43. PMID: 11593028.

CD26-mediated signaling for T cell activation occurs in lipid rafts through its association with CD45RO.

Ponsford et al. 2001. Clin Exp Immunol. 124(2):315-22. PMID: 11422210.

Differential responses of CD45+ve T-cell subsets to MBP in multiple sclerosis.

Smith et al. 1986. Immunology. 58(1):63-70. PMID: 2423439.

Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.


Add a reference


This reagent does not have any reviews at the moment.