Anti-ErbB3 [RTJ1]
Invented at
- Datasheet
- References (14)
- Inventor Info
Info
| Catalogue Number | 151157 |
| Applications | IHC IP WB |
| Antigen/Gene or Protein Targets | ErbB3 (Her3) |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against ErbB3 kinase, widely prevalent in GI and breast tumours. Background and Research Application The EGFR family of type I growth factor receptor tyrosine kinases includes EGFR (HER1), c-erbB2 (HER2; neu), c-erbB3 (HER3) and c-erbB4 (HER4). c-erbB3 is normally found in non-dividing differentiated epithelial cells, neurones and hepatocytes. ErbB3 was found to be kinase impaired, having only 1/1000 the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as an allosteric activator. The kinase-impaired ErbB3 is known to form active heterodimers with other members of the ErbB family, most notably the ligand binding-impaired ErbB2. It has approximately 80% prevalence in a wide variety of tumours of the gastro-intestinal tract, and approximately 20% prevalence in breast cancer. This antibody is specific against c-erbB-3 oncoprotein that is expressed in a variety of human normal, foetal and neoplastic epithelial tissues. |
| Host | Mouse |
| Immunogen | Synthetic Peptide from C terminus of human c-erbB3 |
| Immunogen UniProt ID | P21860 |
| Subclass | IgM |
| Myeloma Used | NS0 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody can be used on formalin-fixed, paraffin-embedded tissue sections and frozen tissue sections. It reacts with normal gastrointestinal epithelium as well as gastrointestinal and breast tumours, resulting in a cytoplasmic staining pattern. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction |
References: 14 entries
Li et al. 2017. Oncotarget. 8(40):67140-67151. PMID: 28978022.
Lattanzio et al. 2016. Oncol Lett. 12(1):453-458. PMID: 27347164.
Anagnostou et al. 2010. Cancer Epidemiol Biomarkers Prev. 19(4):982-91. PMID: 20332259.
Analytic variability in immunohistochemistry biomarker studies.
Europe PMC ID: 20332259
Kapitanović et al. 2000. J Cancer Res Clin Oncol. 126(4):205-11. PMID: 10782893.
Expression of erbB-3 protein in colorectal adenocarcinoma: correlation with poor survival.
Europe PMC ID: 10782893
Naidu et al. 1998. Br J Cancer. 78(10):1385-90. PMID: 9823984.
Expression of c-erbB3 protein in primary breast carcinomas.
Europe PMC ID: 9823984
Srinivasan et al. 1998. J Pathol. 185(3):236-45. PMID: 9771476.
Expression of the c-erbB-4/HER4 protein and mRNA in normal human fetal and adult tissues and in a survey of nine solid tumour types.
Europe PMC ID: 9771476
Rajkumar et al. 1996. Clin Mol Pathol. 49(4):M199-202. PMID: 16696074.
c-erbB3 protein expression in ovarian cancer.
Europe PMC ID: 16696074
Rajkumar et al. 1993. J Pathol. 170(3):271-8. PMID: 8133400.
Expression of the c-erbB-3 protein in gastrointestinal tract tumours determined by monoclonal antibody RTJ1.
Europe PMC ID: 8133400
Add a reference
References: 14 entries
Li et al. 2017. Oncotarget. 8(40):67140-67151. PMID: 28978022.
Lattanzio et al. 2016. Oncol Lett. 12(1):453-458. PMID: 27347164.
Anagnostou et al. 2010. Cancer Epidemiol Biomarkers Prev. 19(4):982-91. PMID: 20332259.
Analytic variability in immunohistochemistry biomarker studies.
Kapitanović et al. 2000. J Cancer Res Clin Oncol. 126(4):205-11. PMID: 10782893.
Expression of erbB-3 protein in colorectal adenocarcinoma: correlation with poor survival.
Naidu et al. 1998. Br J Cancer. 78(10):1385-90. PMID: 9823984.
Expression of c-erbB3 protein in primary breast carcinomas.
Srinivasan et al. 1998. J Pathol. 185(3):236-45. PMID: 9771476.
Expression of the c-erbB-4/HER4 protein and mRNA in normal human fetal and adult tissues and in a survey of nine solid tumour types.
Rajkumar et al. 1996. Clin Mol Pathol. 49(4):M199-202. PMID: 16696074.
c-erbB3 protein expression in ovarian cancer.
Rajkumar et al. 1993. J Pathol. 170(3):271-8. PMID: 8133400.
Expression of the c-erbB-3 protein in gastrointestinal tract tumours determined by monoclonal antibody RTJ1.
Add a reference