Anti-PCNA [PC8]
Invented by David Lane from Karolinska Institutet
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151151 |
| Applications | IHC IP WB |
| Antigen/Gene or Protein Targets | Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein |
| Synonyms | Proliferating Cell Nuclear Antigen; DNA Polymerase Delta Auxiliary Protein; ATLD2 |
| Reactivity | Human, Schizosaccharomyces pombe and Saccharomyces frugiperda |
| Relevance | PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis). |
| Host | Mouse |
| Immunogen | Protein A-PCNA fusion obtained from pC2T. |
| Subclass | IgG3 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | RPMI 1640 + 5% FCS. |
| Notes | The PCNA Epitope also found in Schizosaccharomyces pombe (yeast) and Spodoptera frugiperda (insect) in addition to man. PC8 is recommended for use on formalin-fixed paraffin embedded sections. |
| Research Area | Cancer, Cell Cycle, Cell Type or Organelle Marker, Epigenetics & Nuclear Signalling |
References: 4 entries
Yu et al. 1995. Anal Cell Pathol. 9(1):45-52. PMID: 7577754.
IHC
Optimization of immunohistochemical staining of proliferating cells in paraffin sections of breast carcinoma using antibodies to proliferating cell nuclear antigen and the Ki-67 antigen.
Europe PMC ID: 7577754
Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
Europe PMC ID: 1695635
Add a reference
References: 4 entries
Yu et al. 1995. Anal Cell Pathol. 9(1):45-52. PMID: 7577754.
IHC
Optimization of immunohistochemical staining of proliferating cells in paraffin sections of breast carcinoma using antibodies to proliferating cell nuclear antigen and the Ki-67 antigen.
Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
Add a reference
