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Anti-p53 [Pab 240]

Invented by David Lane at Karolinska Institutet

Info

Catalogue Number 151146
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets p53
Reactivity Chicken and Mammalian
Relevance p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas.
Host Mouse
Immunogen A gel purified p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone)
Subclass IgG1 kappa
Positive Control MDA-MB-231 cell line
Molecular Weight (kDa) 53
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions RPMI 1640 + 7.5% FCS
Strain Balb/c
Notes Detection of p53 using PAb 240: 50% colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissue are negative.
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

References: 17 entries

İlhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and surfactant protein in the lungs of sheep with pulmonary adenomatosis.

Europe PMC ID: 27016721

Jakobsen et al. 2015. J Extracell Vesicles. 4:26659. PMID: 25735706.

Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma.

Europe PMC ID: 25735706

Pan et al. 2009. EMBO J. 28(22):3500-13. PMID: 19745809.

SnoN functions as a tumour suppressor by inducing premature senescence.

Europe PMC ID: 19745809

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Immunohistochemical analysis of p53 expression in malignant lymphomas.

Europe PMC ID: 1466398

Walker et al. 1991. J Pathol. 165(3):203-11. PMID: 1684809.

Expression of p53 protein in infiltrating and in-situ breast carcinomas.

Europe PMC ID: 1684809

Bártek et al. 1991. Oncogene. 6(9):1699-703. PMID: 1923535.

Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies.

Europe PMC ID: 1923535

Gannon et al. 1990. EMBO J. 9(5):1595-602. PMID: 1691710.

Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form.

Europe PMC ID: 1691710

Iggo et al. 1990. Lancet. 335(8691):675-9. PMID: 1969059.

Increased expression of mutant forms of p53 oncogene in primary lung cancer.

Europe PMC ID: 1969059

Greaves. R. F (1988) Ph.D Thesis. University of London.


Add a reference

References: 17 entries

İlhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and surfactant protein in the lungs of sheep with pulmonary adenomatosis.

Europe PMC ID: 27016721

Jakobsen et al. 2015. J Extracell Vesicles. 4:26659. PMID: 25735706.

Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma.

Europe PMC ID: 25735706

Pan et al. 2009. EMBO J. 28(22):3500-13. PMID: 19745809.

SnoN functions as a tumour suppressor by inducing premature senescence.

Europe PMC ID: 19745809

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Immunohistochemical analysis of p53 expression in malignant lymphomas.

Europe PMC ID: 1466398

Walker et al. 1991. J Pathol. 165(3):203-11. PMID: 1684809.

Expression of p53 protein in infiltrating and in-situ breast carcinomas.

Europe PMC ID: 1684809

Bártek et al. 1991. Oncogene. 6(9):1699-703. PMID: 1923535.

Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies.

Europe PMC ID: 1923535

Gannon et al. 1990. EMBO J. 9(5):1595-602. PMID: 1691710.

Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form.

Europe PMC ID: 1691710

Iggo et al. 1990. Lancet. 335(8691):675-9. PMID: 1969059.

Increased expression of mutant forms of p53 oncogene in primary lung cancer.

Europe PMC ID: 1969059

Greaves. R. F (1988) Ph.D Thesis. University of London.


Add a reference

References: 17 entries

İlhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and surfactant protein in the lungs of sheep with pulmonary adenomatosis.

Jakobsen et al. 2015. J Extracell Vesicles. 4:26659. PMID: 25735706.

Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma.

Pan et al. 2009. EMBO J. 28(22):3500-13. PMID: 19745809.

SnoN functions as a tumour suppressor by inducing premature senescence.

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Immunohistochemical analysis of p53 expression in malignant lymphomas.

Walker et al. 1991. J Pathol. 165(3):203-11. PMID: 1684809.

Expression of p53 protein in infiltrating and in-situ breast carcinomas.

Bártek et al. 1991. Oncogene. 6(9):1699-703. PMID: 1923535.

Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies.

Gannon et al. 1990. EMBO J. 9(5):1595-602. PMID: 1691710.

Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form.

Iggo et al. 1990. Lancet. 335(8691):675-9. PMID: 1969059.

Increased expression of mutant forms of p53 oncogene in primary lung cancer.

Greaves. R. F (1988) Ph.D Thesis. University of London.


Add a reference


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