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|Applications||ChIP ELISA FACS IHC IF IP RIA WB|
|Antigen/Gene or Protein Targets||p53|
|Relevance||p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. PAb 1801 can be used for the specific detection of human p53 that is synthesised in the presence of p53 from other species.|
|Immunogen||Purified p53-b-galactosidase fusion proteins|
|Positive Control||MDA-MB-231 cell line|
|Molecular Weight (kDa)||53|
|Recommended Growing Conditions||RPMI + 10% FCS|
|Notes||PAb 1801 may be used for the specific detection of human p53 which is synthesised in the presence of p53 from other species and is suitable for immunohistochemistry using paraffin embedded samples with pepsin treatment. pAb1801,which recognizes an epitope between amino acids 32 and 79 of both wild-type and mutant p53 (from PMID:12692844)|
|Research Area||Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling|
References: 8 entries
Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.
Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.
Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.
Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.
Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.
Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.
Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.
Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.
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