Cancer Research Technology
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Catalogue Number 151144
Applications ChIP ELISA FACS IHC IF IP RIA WB
Antigen/Gene or Protein Targets p53
Reactivity Human
Relevance p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. PAb 1801 can be used for the specific detection of human p53 that is synthesised in the presence of p53 from other species.
Host Mouse
Immunogen Purified p53-b-galactosidase fusion proteins
Subclass IgG1
Positive Control MDA-MB-231 cell line
Molecular Weight (kDa) 53
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS
Strain Balb/c
Notes PAb 1801 may be used for the specific detection of human p53 which is synthesised in the presence of p53 from other species and is suitable for immunohistochemistry using paraffin embedded samples with pepsin treatment. pAb1801,which recognizes an epitope between amino acids 32 and 79 of both wild-type and mutant p53 (from PMID:12692844)

Please note that there are some publications that suggest the PAb 1801 clone also cross reacts proteins unrelated to p53 for example an unknown component of processing bodies (P-bodies) (Wolf et al., 2001. Oncogene. 15;20(11):1307-17. PMID: 11313875; Thomas et al., 2012. PLoS One. 2012; 7(5): e36447 PMID: 22590546). Please ensure that appropriate controls (e.g. MW ladder, treatment to induce p53 expression) are included to ensure the positive signal is from p53.
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

References: 20 entries

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Lysines in the tetramerization domain of p53 selectively modulate G1 arrest.

Europe PMC ID: 27210019

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP

Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters.

Europe PMC ID: 24928858

Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.

p53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (ER) stress.

Europe PMC ID: 23341460

Thomas et al. 2012. PLoS One. 7(5):e36447. PMID: 22590546.

A monoclonal antibody against p53 cross-reacts with processing bodies.

Europe PMC ID: 22590546

Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.

Can p53 staining be used to identify patients with aggressive superficial bladder cancer?

Europe PMC ID: 12692844

Wolff et al. 2001. Oncogene. 20(11):1307-17. PMID: 11313875.

Evidence that wild-type p53 in neuroblastoma cells is in a conformation refractory to integration into the transcriptional complex.

Europe PMC ID: 11313875

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Immunohistochemical analysis of p53 expression in malignant lymphomas.

Europe PMC ID: 1466398

Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.

Widespread p53 overexpression in human malignant tumors. An immunohistochemical study using methacarn-fixed, embedded tissue.

Europe PMC ID: 1731521

Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.

P53 expression in breast cancer.

Europe PMC ID: 3276632

Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.

Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression.

Europe PMC ID: 2428616


Add a reference

References: 20 entries

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Lysines in the tetramerization domain of p53 selectively modulate G1 arrest.

Europe PMC ID: 27210019

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP

Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters.

Europe PMC ID: 24928858

Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.

p53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (ER) stress.

Europe PMC ID: 23341460

Thomas et al. 2012. PLoS One. 7(5):e36447. PMID: 22590546.

A monoclonal antibody against p53 cross-reacts with processing bodies.

Europe PMC ID: 22590546

Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.

Can p53 staining be used to identify patients with aggressive superficial bladder cancer?

Europe PMC ID: 12692844

Wolff et al. 2001. Oncogene. 20(11):1307-17. PMID: 11313875.

Evidence that wild-type p53 in neuroblastoma cells is in a conformation refractory to integration into the transcriptional complex.

Europe PMC ID: 11313875

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Immunohistochemical analysis of p53 expression in malignant lymphomas.

Europe PMC ID: 1466398

Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.

Widespread p53 overexpression in human malignant tumors. An immunohistochemical study using methacarn-fixed, embedded tissue.

Europe PMC ID: 1731521

Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.

P53 expression in breast cancer.

Europe PMC ID: 3276632

Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.

Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression.

Europe PMC ID: 2428616


Add a reference

References: 20 entries

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Lysines in the tetramerization domain of p53 selectively modulate G1 arrest.

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP

Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters.

Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.

p53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (ER) stress.

Thomas et al. 2012. PLoS One. 7(5):e36447. PMID: 22590546.

A monoclonal antibody against p53 cross-reacts with processing bodies.

Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.

Can p53 staining be used to identify patients with aggressive superficial bladder cancer?

Wolff et al. 2001. Oncogene. 20(11):1307-17. PMID: 11313875.

Evidence that wild-type p53 in neuroblastoma cells is in a conformation refractory to integration into the transcriptional complex.

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Immunohistochemical analysis of p53 expression in malignant lymphomas.

Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.

Widespread p53 overexpression in human malignant tumors. An immunohistochemical study using methacarn-fixed, embedded tissue.

Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.

P53 expression in breast cancer.

Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.

Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression.


Add a reference


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