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Catalogue Number 151144
Applications
RECENTLY UPDATED
ChIP ELISA FACS IHC IF IP RIA WB
Antigen/Gene or Protein Targets p53
Reactivity Human
Relevance p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. PAb 1801 can be used for the specific detection of human p53 that is synthesised in the presence of p53 from other species.
Host Mouse
Immunogen Purified p53-b-galactosidase fusion proteins
Subclass IgG1
Positive Control MDA-MB-231 cell line
Molecular Weight (kDa) 53
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS
Strain Balb/c
Notes
RECENTLY UPDATED
PAb 1801 may be used for the specific detection of human p53 which is synthesised in the presence of p53 from other species and is suitable for immunohistochemistry using paraffin embedded samples with pepsin treatment. pAb1801,which recognizes an epitope between amino acids 32 and 79 of both wild-type and mutant p53 (from PMID:12692844)

Please note that there are some publications that suggest the PAb 1801 clone also cross reacts proteins unrelated to p53 for example an unknown component of processing bodies (P-bodies) (Wolf et al., 2001. Oncogene. 15;20(11):1307-17. PMID: 11313875; Thomas et al., 2012. PLoS One. 2012; 7(5): e36447 PMID: 22590546). Please ensure that appropriate controls (e.g. MW ladder, treatment to induce p53 expression) are included to ensure the positive signal is from p53.
Research Area
RECENTLY UPDATED
Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

References: 10 entries
RECENTLY UPDATED

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP

Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.

Thomas et al. 2012. PLoS One. 7(5):e36447. PMID: 22590546.

Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.

Wolff et al. 2001. Oncogene. 20(11):1307-17. PMID: 11313875.

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.

Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.

Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.


Add a reference

References: 10 entries
RECENTLY UPDATED

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP

Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.

Thomas et al. 2012. PLoS One. 7(5):e36447. PMID: 22590546.

Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.

Wolff et al. 2001. Oncogene. 20(11):1307-17. PMID: 11313875.

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.

Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.

Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.


Add a reference

References: 10 entries
RECENTLY UPDATED

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP

Thomas et al. 2013. J Biol Chem. 288(11):7606-17. PMID: 23341460.

Thomas et al. 2012. PLoS One. 7(5):e36447. PMID: 22590546.

Masters et al. 2003. J Pathol. 200(1):74-81. PMID: 12692844.

Wolff et al. 2001. Oncogene. 20(11):1307-17. PMID: 11313875.

Said et al. 1992. Am J Pathol. 141(6):1343-8. PMID: 1466398.

Porter et al. 1992. Am J Pathol. 140(1):145-53. PMID: 1731521.

Cattoretti et al. 1988. Int J Cancer. 41(2):178-83. PMID: 3276632.

Banks et al. 1986. Eur J Biochem. 159(3):529-34. PMID: 2428616.


Add a reference


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