Anti-Integrin alpha V [P2W7]
Invented at King's College London
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151140 |
| Applications | FACS IHC IF IP WB |
| Antigen/Gene or Protein Targets | Integrin alpha V (CD51) |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which detects the ectodomain of integrin aV. Background and Research Application Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin aV associates with integrin b3 to form the osteoclast functional antigen (vitronectin receptor). The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF Integrin aV is found in osteoclasts, placenta, melanoma cell lines and some epithelial cell lines. Expression of integrin aV increases with melanoma progression. Anti-integrin aV detects the ectodomain of human integrin alpha V/CD51. |
| Host | Mouse |
| Immunogen | Ocular melanoma cell line (V+B2) a clone expressing high levels of alpha-v, beta-1 |
| Immunogen UniProt ID | P06756 |
| Positive Control | A375M melanoma |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 150-160 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Notes |
Production Details Purified using multi-step affinity chromatography. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa. Overexpression of the integrin aV is associated with progression and spread of colorectal cancer, and prostate cancer. Concentration 1mg/ml as standard |
| Research Area | Stem Cell Biology |
References: 6 entries
D'Agostino et al. 2020. Molecules. 25(3):. PMID: 31972968.
Stantchev et al. 2019. Viruses. 11(10):. PMID: 31547585.
Murase et al. 2017. J Neurosci Res. :. PMID: 28509387.
IF WB
Richards et al. 2011. J Virol. 85(17):8766-76. PMID: 21715474.
Misselwitz et al. 2011. Mol Syst Biol. 7:474. PMID: 21407211.
von Schlippe et al. 2000. J Cell Sci. 113 ( Pt 3):425-37. PMID: 10639330.
Add a reference
References: 6 entries
D'Agostino et al. 2020. Molecules. 25(3):. PMID: 31972968.
Stantchev et al. 2019. Viruses. 11(10):. PMID: 31547585.
Murase et al. 2017. J Neurosci Res. :. PMID: 28509387.
IF WB
Richards et al. 2011. J Virol. 85(17):8766-76. PMID: 21715474.
Misselwitz et al. 2011. Mol Syst Biol. 7:474. PMID: 21407211.
von Schlippe et al. 2000. J Cell Sci. 113 ( Pt 3):425-37. PMID: 10639330.
Add a reference
