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Anti-Integrin alpha V [P2W7]

Info

Catalogue Number 151140
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Integrin alpha V (CD51)
Reactivity Human
Relevance Monoclonal antibody which detects the ectodomain of integrin aV.

Background and Research Application
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin aV associates with integrin b3 to form the osteoclast functional antigen (vitronectin receptor). The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF
Integrin aV is found in osteoclasts, placenta, melanoma cell lines and some epithelial cell lines. Expression of integrin aV increases with melanoma progression. Anti-integrin aV detects the ectodomain of human integrin alpha V/CD51.
Host Mouse
Immunogen Ocular melanoma cell line (V+B2) a clone expressing high levels of alpha-v, beta-1
Immunogen UniProt ID P06756
Positive Control A375M melanoma
Subclass IgG1
Molecular Weight (kDa) 150-160
Recommended Growing Conditions DMEM + 5% FCS
Notes Production Details
Purified using multi-step affinity chromatography.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Human alpha v is about 150-160kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130kDa +20-25kDa.

Overexpression of the integrin aV is associated with progression and spread of colorectal cancer, and prostate cancer.

Concentration
1mg/ml as standard
Research Area Stem Cell Biology

References

There are 6 reference entries for this reagent.

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References: 6 entries

D'Agostino et al. 2020. Molecules. 25(3):. PMID: 31972968.

Stantchev et al. 2019. Viruses. 11(10):. PMID: 31547585.

Murase et al. 2017. J Neurosci Res. :. PMID: 28509387.

IF WB

Richards et al. 2011. J Virol. 85(17):8766-76. PMID: 21715474.

Misselwitz et al. 2011. Mol Syst Biol. 7:474. PMID: 21407211.

von Schlippe et al. 2000. J Cell Sci. 113 ( Pt 3):425-37. PMID: 10639330.


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References: 6 entries

D'Agostino et al. 2020. Molecules. 25(3):. PMID: 31972968.

Stantchev et al. 2019. Viruses. 11(10):. PMID: 31547585.

Murase et al. 2017. J Neurosci Res. :. PMID: 28509387.

IF WB

Richards et al. 2011. J Virol. 85(17):8766-76. PMID: 21715474.

Misselwitz et al. 2011. Mol Syst Biol. 7:474. PMID: 21407211.

von Schlippe et al. 2000. J Cell Sci. 113 ( Pt 3):425-37. PMID: 10639330.


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Inventor Information

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