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Anti-Keratin8 [LP3K]

Invented by Prof Birgit Lane at A*STAR

Info

Catalogue Number 151126
Applications IHC IF WB
Antigen/Gene or Protein Targets Keratin 8
Reactivity Human
Relevance Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Keratin 8 is the tissue polypeptide antigen (TPA) reported to be present in the serum of cancer patients.
Host Mouse
Immunogen Sonicated cytoskeleton fractions (insoluble in 1% Nonidet-P40) from SV40 transformed neonatal keratinocytes.
Subclass IgG1
Molecular Weight (kDa) 52
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Research Area Cell Signaling & Signal Transduction

References: 6 entries

Waseem et al. 2004. Biochemistry. 43(5):1283-95. PMID: 14756564.

Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability.

Europe PMC ID: 14756564

Godsave et al. 1986. J Embryol Exp Morphol. 97:201-23. PMID: 2432146.

The appearance and distribution of intermediate filament proteins during differentiation of the central nervous system, skin and notochord of Xenopus laevis.

Europe PMC ID: 2432146

Lane et al. 1985. Ann N Y Acad Sci. 455:241-58. PMID: 2417515.

Keratin antigens in differentiating skin.

Europe PMC ID: 2417515


Add a reference

References: 6 entries

Waseem et al. 2004. Biochemistry. 43(5):1283-95. PMID: 14756564.

Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability.

Europe PMC ID: 14756564

Godsave et al. 1986. J Embryol Exp Morphol. 97:201-23. PMID: 2432146.

The appearance and distribution of intermediate filament proteins during differentiation of the central nervous system, skin and notochord of Xenopus laevis.

Europe PMC ID: 2432146

Lane et al. 1985. Ann N Y Acad Sci. 455:241-58. PMID: 2417515.

Keratin antigens in differentiating skin.

Europe PMC ID: 2417515


Add a reference

References: 6 entries

Waseem et al. 2004. Biochemistry. 43(5):1283-95. PMID: 14756564.

Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability.

Godsave et al. 1986. J Embryol Exp Morphol. 97:201-23. PMID: 2432146.

The appearance and distribution of intermediate filament proteins during differentiation of the central nervous system, skin and notochord of Xenopus laevis.

Lane et al. 1985. Ann N Y Acad Sci. 455:241-58. PMID: 2417515.

Keratin antigens in differentiating skin.


Add a reference


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