Anti-Keratin7 [LP1K]
Invented by Prof Birgit Lane from A*STAR Accelerate Technologies Pte Ltd
Invented at Queen Mary University of London
- Datasheet
- References (11)
- Inventor Info
Info
![Image thumbnail for Anti-Keratin7 [LP1K]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/4fe88947-d7b2-4016-9627-0d77843cd9db.jpg)
| Catalogue Number | 151124 |
| Applications | IHC IF WB |
| Antigen/Gene or Protein Targets | Keratin 7 |
| Reactivity | Mammalian and Xenopus laevis |
| Relevance |
Monoclonal antibody which binds keratin 7. Background and Research Application Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 7 and 8 are two closely related type II keratins characteristic of simple epithelia. Keratin 7 is less widespread than keratin 8 and is expressed in sebaceous and sweat glands, blood vessels and some cells of the inner hair root sheath. Keratin 7 is often co-expressed with keratin 19. LP1K has some weak reactivity with keratin 8 and other type II keratins. |
| Host | Mouse |
| Immunogen | Sonicated cytoskeleton fractions (insoluble in 1% Nonidet-P40) from SV40 transformed neonatal keratinocytes. |
| Immunogen UniProt ID | P08729 |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 54 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest As the keratin 7 antigen is found in both healthy and neoplastic cells, antibodies to K7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively. Not recommended for fixed tissue immunohistochemistry. Concentration 1mg/ml as standard |
| Research Area | Cell Signaling & Signal Transduction |
References: 11 entries
Lane EB et al. 1985. Ann. NY Acad. Sci. 455:241-58.PMID: 2417515
Reyes et al. 2018. J Oral Microbiol. 10(1):1374153. PMID: 29291034.
Yougbaré et al. 2017. Nat Commun. 8(1):224. PMID: 28794456.
Devos et al. 2017. J Invest Dermatol. 137(2):494-505. PMID: 27725202.
Zhang et al. 2015. Reproduction. 150(2):139-49. PMID: 26021998.
Zhang et al. 2013. Cell Death Dis. 4:e502. PMID: 23429288.
CUL1 promotes trophoblast cell invasion at the maternal-fetal interface.
Europe PMC ID: 23429288
Zhang et al. 2012. PLoS One. 7(6):e38487. PMID: 22679510.
Expression of CD82 in human trophoblast and its role in trophoblast invasion.
Europe PMC ID: 22679510
Markey et al. 1992. Br J Dermatol. 126(2):154-60. PMID: 1371396.
Keratin expression in basal cell carcinomas.
Europe PMC ID: 1371396
Add a reference
References: 11 entries
Lane EB et al. 1985. Ann. NY Acad. Sci. 455:241-58.PMID: 2417515
Reyes et al. 2018. J Oral Microbiol. 10(1):1374153. PMID: 29291034.
Yougbaré et al. 2017. Nat Commun. 8(1):224. PMID: 28794456.
Devos et al. 2017. J Invest Dermatol. 137(2):494-505. PMID: 27725202.
Zhang et al. 2015. Reproduction. 150(2):139-49. PMID: 26021998.
Zhang et al. 2013. Cell Death Dis. 4:e502. PMID: 23429288.
CUL1 promotes trophoblast cell invasion at the maternal-fetal interface.
Zhang et al. 2012. PLoS One. 7(6):e38487. PMID: 22679510.
Expression of CD82 in human trophoblast and its role in trophoblast invasion.
Markey et al. 1992. Br J Dermatol. 126(2):154-60. PMID: 1371396.
Keratin expression in basal cell carcinomas.
Add a reference
