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Anti-Keratin7 [LP1K]

Invented by Prof Birgit Lane at Queen Mary University of London

Info

Catalogue Number 151124
Applications IHC IF WB
Antigen/Gene or Protein Targets Keratin 7
Reactivity Mammalian and Xenopus laevis
Relevance Monoclonal antibody which binds keratin 7.

Background and Research Application
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 7 and 8 are two closely related type II keratins characteristic of simple epithelia. Keratin 7 is less widespread than keratin 8 and is expressed in sebaceous and sweat glands, blood vessels and some cells of the inner hair root sheath. Keratin 7 is often co-expressed with keratin 19.

LP1K has some weak reactivity with keratin 8 and other type II keratins.
Host Mouse
Immunogen Sonicated cytoskeleton fractions (insoluble in 1% Nonidet-P40) from SV40 transformed neonatal keratinocytes.
Immunogen UniProt ID P08729
Subclass IgG1
Molecular Weight (kDa) 54
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
As the keratin 7 antigen is found in both healthy and neoplastic cells, antibodies to K7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively.

Not recommended for fixed tissue immunohistochemistry.

Concentration
1mg/ml as standard
Research Area Cell Signaling & Signal Transduction

References

There are 11 reference entries for this reagent.

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References: 11 entries

Lane EB et al. 1985. Ann. NY Acad. Sci. 455:241-58.PMID: 2417515

Reyes et al. 2018. J Oral Microbiol. 10(1):1374153. PMID: 29291034.

Yougbaré et al. 2017. Nat Commun. 8(1):224. PMID: 28794456.

Devos et al. 2017. J Invest Dermatol. 137(2):494-505. PMID: 27725202.

Zhang et al. 2015. Reproduction. 150(2):139-49. PMID: 26021998.

Zhang et al. 2013. Cell Death Dis. 4:e502. PMID: 23429288.

CUL1 promotes trophoblast cell invasion at the maternal-fetal interface.

Europe PMC ID: 23429288

Zhang et al. 2012. PLoS One. 7(6):e38487. PMID: 22679510.

Expression of CD82 in human trophoblast and its role in trophoblast invasion.

Europe PMC ID: 22679510

Markey et al. 1992. Br J Dermatol. 126(2):154-60. PMID: 1371396.

Keratin expression in basal cell carcinomas.

Europe PMC ID: 1371396


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References: 11 entries

Lane EB et al. 1985. Ann. NY Acad. Sci. 455:241-58.PMID: 2417515

Reyes et al. 2018. J Oral Microbiol. 10(1):1374153. PMID: 29291034.

Yougbaré et al. 2017. Nat Commun. 8(1):224. PMID: 28794456.

Devos et al. 2017. J Invest Dermatol. 137(2):494-505. PMID: 27725202.

Zhang et al. 2015. Reproduction. 150(2):139-49. PMID: 26021998.

Zhang et al. 2013. Cell Death Dis. 4:e502. PMID: 23429288.

CUL1 promotes trophoblast cell invasion at the maternal-fetal interface.

Zhang et al. 2012. PLoS One. 7(6):e38487. PMID: 22679510.

Expression of CD82 in human trophoblast and its role in trophoblast invasion.

Markey et al. 1992. Br J Dermatol. 126(2):154-60. PMID: 1371396.

Keratin expression in basal cell carcinomas.


Add a reference

Inventor Information